CAJ | 학술논문

目的：研究Survivin、Livin基因共沉默对前列腺癌细胞PC-3在细胞周期、细胞增殖、细胞凋亡、对化疗药物敏感性、克隆形成及裸鼠体内成瘤等方面的影响以及Survivin shRNA、Livin shRNA联合促前列腺癌细胞凋亡作用。方法：运用分子克隆技术，构建以Survivin、Livin基因为靶点的、含Survivin启动子的CGM30 miR
목적：연구Survivin、Livin기인공침묵대전렬선암세포PC-3재세포주기、세포증식、세포조망、대화료약물민감성、극륭형성급라서체내성류등방면적영향이급Survivin shRNA、Livin shRNA연합촉전렬선암세포조망작용。방법：운용분자극륭기술，구건이Survivin、Livin기인위파점적、함Survivin계동자적CGM30 miR
Objective:To observe the antitumor effects of the tumor specificity RNAi recombinated lentivirus which content the Survivin promoter and Survivin shRNA and Livin shRNA on human prostate cancer cell PC-3. Methods:The CMV promoter of CGM30 miR-30 shRNA vector was replaced by Survivin promoter,then two target gene seg-ments were synthesized and cloned into CGM30 miR-30 shRNA vector respectively. Recombinant vectors was cont-ranfected into 293T cells with pHelper 1. 0 and pHelper 2. 0 plasmid to get packaged recombinant lentivirus. Then PClot. The apoptosis index and cells cycle of PC -3 cells was detected by Flow cytometry. The survival curve of different group PC -3 cells were detected by MTT method. To observe the tumor formation and grown of PC -3 cells which treated by recombinant lentivirus in nudemice xenografts.Results:The results of RT - PCR and Western blot indicated that recombinant lentivirus could knock down the transcription and expression of Survivin and Livin gene only in PC -3 cells. After treated with recombinant lentivirus,the apoptosis index of PC -3 cells was increased about 20%, the number of PC -3 cells of S phase was increase about 5% - 10%,the mean volume and weight of tumors which treated with recombinant lentivirus were less than 50% compared with control groups. Conclusion:Recombinant lentivirus mediated downregulation of Survivin and Livin expression can lead to potent antitumor activity and chemosensitizing effects in prostate cancer not only in vivo but also in vitro,and the antitumor activity and chemosensitizing effects was efficiently then downregulation of Survivin or Livin expression in PC -3 cells unitary.