现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2014年
6期
1261-1264
,共4页
赵成志%毕芳芳%王光伟%杨清
趙成誌%畢芳芳%王光偉%楊清
조성지%필방방%왕광위%양청
子宫内膜癌%PARP-1%免疫组化SP法%RT-PCR
子宮內膜癌%PARP-1%免疫組化SP法%RT-PCR
자궁내막암%PARP-1%면역조화SP법%RT-PCR
endometrial carcinoma%PARP-1%SP%RT-PCR
目的：研究不同浓度孕激素作用子宫内膜癌Ishikawa细胞后DNA损伤修复蛋白PARP-1的表达变化，探讨PARP-1在子宫内膜腺癌中的作用及PARP-1和孕激素相互关系。方法：免疫组化SP法和实时荧光定量PCR（Real-time PCR）法检测不同浓度的醋酸甲羟孕酮（Medroxyprogesterone acetate，MPA）干预Ish-ikawa细胞后PARP-1蛋白及PARP-1 mRNA表达的变化。结果：免疫组化显示PARP-1蛋白表达于Ish-ikawa细胞核，随着MPA浓度的增加PARP-1的表达呈下降趋势。RT-PCR检测到MPA呈浓度依赖性地抑制PARP-1 mRNA表达。结论：醋酸甲羟孕酮呈浓度依赖性地抑制PARP-1的表达，提示孕激素可能间接或直接通过抑制PARP-1而发挥治疗子宫内膜癌的作用。
目的：研究不同濃度孕激素作用子宮內膜癌Ishikawa細胞後DNA損傷脩複蛋白PARP-1的錶達變化，探討PARP-1在子宮內膜腺癌中的作用及PARP-1和孕激素相互關繫。方法：免疫組化SP法和實時熒光定量PCR（Real-time PCR）法檢測不同濃度的醋痠甲羥孕酮（Medroxyprogesterone acetate，MPA）榦預Ish-ikawa細胞後PARP-1蛋白及PARP-1 mRNA錶達的變化。結果：免疫組化顯示PARP-1蛋白錶達于Ish-ikawa細胞覈，隨著MPA濃度的增加PARP-1的錶達呈下降趨勢。RT-PCR檢測到MPA呈濃度依賴性地抑製PARP-1 mRNA錶達。結論：醋痠甲羥孕酮呈濃度依賴性地抑製PARP-1的錶達，提示孕激素可能間接或直接通過抑製PARP-1而髮揮治療子宮內膜癌的作用。
목적：연구불동농도잉격소작용자궁내막암Ishikawa세포후DNA손상수복단백PARP-1적표체변화，탐토PARP-1재자궁내막선암중적작용급PARP-1화잉격소상호관계。방법：면역조화SP법화실시형광정량PCR（Real-time PCR）법검측불동농도적작산갑간잉동（Medroxyprogesterone acetate，MPA）간예Ish-ikawa세포후PARP-1단백급PARP-1 mRNA표체적변화。결과：면역조화현시PARP-1단백표체우Ish-ikawa세포핵，수착MPA농도적증가PARP-1적표체정하강추세。RT-PCR검측도MPA정농도의뢰성지억제PARP-1 mRNA표체。결론：작산갑간잉동정농도의뢰성지억제PARP-1적표체，제시잉격소가능간접혹직접통과억제PARP-1이발휘치료자궁내막암적작용。
Objective:To study the effect of PARP-1 expression of Ishikawa cells treated with different concen-tration of MPA,discussed the role of PARP-1 in the development of endometrial cancer,and the mutual relations be-tween PARP-1 and progesterone. Methods:The expression of PARP-1 was detected by immunohistochemical tech-nique,and PARP-1 mRNA by real-time PCR. Results:Immunohistochemistry showed that PARP-1 protein ex-pression in Ishikawa cell nucleus,with the increase of the concentration of MPA PARP-1 expression showed a down-ward trend. RT-PCR detected MPA concentration -dependent inhibition of PARP -1mRNA expression. Conclu-sion:Medroxyprogesterone acetate can affect DNA repair protein PARP-1 expression,to some extent,inhibited the expression of PARP-1 showed a concentration dependent,and also suggest progesterone may directly or indirectly by inhibiting PARP-1 play a role in the treatment of endometrial cancer.
