CAJ | 학술논문

目的：检测血管能抑素（ canstatin）对甲状腺癌SW579细胞的增殖抑制、诱导凋亡与衰老作用及其机制。方法：利用软琼脂克隆形成实验检测细胞增殖。分别利用Annexin V-FITC/PI双染和β-半乳糖苷酶染色检测细胞凋亡和衰老。Western blot技术检测可能的机制。结果：Canstatin可以明显抑制SW579细胞增殖，诱导其凋亡和衰老。处理后细胞的p-AKT蛋白水平明显下降。而caspase 3和9蛋白水平升高。结论：Can-statin通过抑制p-AKT导致SW579细胞凋亡和衰老。
목적：검측혈관능억소（ canstatin）대갑상선암SW579세포적증식억제、유도조망여쇠로작용급기궤제。방법：이용연경지극륭형성실험검측세포증식。분별이용Annexin V-FITC/PI쌍염화β-반유당감매염색검측세포조망화쇠로。Western blot기술검측가능적궤제。결과：Canstatin가이명현억제SW579세포증식，유도기조망화쇠로。처리후세포적p-AKT단백수평명현하강。이caspase 3화9단백수평승고。결론：Can-statin통과억제p-AKT도치SW579세포조망화쇠로。
Objective:To detect the effects and mechanisms of canstatin on thyroid cancer cell SW579. Methods:The proliferative ratio of SW579 cells after treated with canstatin was detected by soft agarose assay. Annexin-V/FITC and PI double -staining and SA -β -gal staining were used to detect the apoptosis and the senescence of SW579 cells after treated with canstatin,respectively. The possible mechanisms were analyzed by Western-blot. Re-sults:Canstatin could inhibit the proliferation and induce apoptosis and senescence in SW579 cells. The level of p-AKT was lower in treated cells than untreated ones. The levels of caspase 3 and 9 expression were up-regulated in SW579 cells by canstatin. Conclusion:Canstatin could induce apoptosis and senescence in SW579 cells by suppress-ing p-AKT.