CAJ | 학술논문

目的：探讨ING4基因在表达上调的卵巢上皮性癌（卵巢癌）细胞系OVCAR细胞中对顺铂耐药性的影响。方法：脂质体介导的ING4-Ad及相应阴性对照片段转染人卵巢癌细胞OVCAR，实验分为3组：转染组、阴性对照组、空白对照组。实时荧光定量PCR技术检测3组细胞中ING4基因的表达；采用四甲基偶氮唑蓝（ MTT）法测定各组细胞增殖抑制率和绘制细胞生长曲线，集落形成实验测定细胞生长能力，检测3组细胞对顺铂的敏感性，及不同浓度顺铂和作用不同时间后3组细胞生长的抑制率和凋亡率及细胞周期变化。结果：转染组、阴性对照组及空白对照组OVCAR细胞中ING4的表达量分别为66.76±11.40、1.28±0.09、1.22
목적：탐토ING4기인재표체상조적란소상피성암（란소암）세포계OVCAR세포중대순박내약성적영향。방법：지질체개도적ING4-Ad급상응음성대조편단전염인란소암세포OVCAR，실험분위3조：전염조、음성대조조、공백대조조。실시형광정량PCR기술검측3조세포중ING4기인적표체；채용사갑기우담서람（ MTT）법측정각조세포증식억제솔화회제세포생장곡선，집락형성실험측정세포생장능력，검측3조세포대순박적민감성，급불동농도순박화작용불동시간후3조세포생장적억제솔화조망솔급세포주기변화。결과：전염조、음성대조조급공백대조조OVCAR세포중ING4적표체량분별위66.76±11.40、1.28±0.09、1.22
Objective:To investigate the ING4 gene upregulation of epithelial ovarian cancer( ovarian cancer )on cisplatin -resistant cell lines OVCAR cells. Methods:The liposome -mediated ING4 -Ad and the corresponding negative control fragment was transfected into human ovarian cancer OVCAR,rats were divided into three groups:transfection group,negative control group and blank control group. Real-time PCR technology was used to detect the expression of ING4 gene. Using tetrazolium blue( MTT)assay to make inhibition of cell proliferation and cell growth curve,colony formation assay measured the growth of the cells' ability. Results:In transfection group,negative control group and blank control group ING4 expression of OVCAR cells were 66. 76 ± 11. 40,1. 28 ± 0. 09,1. 22 ± 0. 08 (P<0. 01). MTT assay showed that the growth rate slowed transfected cells,decreased sensitivity to cisplatin,in transfected cell growth inhibition rate was the lowest(P<0. 01). Transfected cells grow slowly compared with the neg-ative control group,set transfected cell colony formation rate is lower than the negative control group and blank control group(P<0. 05). Flow cytometry showed that the three groups with the extension of the apoptosis rate increased con-centrations of cisplatin(P<0. 05). Conclusion:The ovarian OVCAR cells ING4 gene,inhibit cell proliferation,apop-tosis decreased sensitivity to cisplatin.