江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
6期
81-83
,共3页
高效液相色谱法%烟叶%没食子酸%萃取
高效液相色譜法%煙葉%沒食子痠%萃取
고효액상색보법%연협%몰식자산%췌취
HPLC%Tobacco leaf%Galic acid%Extraction
建立了高效液相色谱( HPLC)测定烟叶中没食子酸含量的方法。以超纯水为萃取剂,样品经超声提取后,采用C18色谱柱分离/二极管阵列检测器测定。结果表明:没食子酸在0.5~50.0 mg/L范围内的线性相关系数为0.9999,检出限为1.07μg/g,加标回收率为92.7%~101.6%,RSD<5%(n=5)。9个烟叶样品没食子酸含量在107.0~242.8μg/g之间;不同部位烟叶没食子酸含量排序为:上部烟叶>中部烟叶>下部烟叶。
建立瞭高效液相色譜( HPLC)測定煙葉中沒食子痠含量的方法。以超純水為萃取劑,樣品經超聲提取後,採用C18色譜柱分離/二極管陣列檢測器測定。結果錶明:沒食子痠在0.5~50.0 mg/L範圍內的線性相關繫數為0.9999,檢齣限為1.07μg/g,加標迴收率為92.7%~101.6%,RSD<5%(n=5)。9箇煙葉樣品沒食子痠含量在107.0~242.8μg/g之間;不同部位煙葉沒食子痠含量排序為:上部煙葉>中部煙葉>下部煙葉。
건립료고효액상색보( HPLC)측정연협중몰식자산함량적방법。이초순수위췌취제,양품경초성제취후,채용C18색보주분리/이겁관진렬검측기측정。결과표명:몰식자산재0.5~50.0 mg/L범위내적선성상관계수위0.9999,검출한위1.07μg/g,가표회수솔위92.7%~101.6%,RSD<5%(n=5)。9개연협양품몰식자산함량재107.0~242.8μg/g지간;불동부위연협몰식자산함량배서위:상부연협>중부연협>하부연협。
A method for the determination of galic acid content in tobacco leaves was developed by using HPLC ( High Perform-ance Liquid Chromatography ) .After ultrasonic extraction by ultrapure water , the samples were separated by C 18 chromatographic column, and were detected by diode array detector .The results showed that:within the scope of 0.5~50.0 mg/L galic acid con-tent, the linear correlation coefficient was 0.9999, the low detection limit was 1.07μg/g, the standard-adding recovery was from 92.7%to 101.6%, and the RSD was less than 5% (n=5).The contents of galic acid in 9 tobacco leaf samples ranged from 107.0 to 242.8 μg/g.The galic acid content in different parts of tobacco leaves could be arranged as follows :upper leaf >middle leaf >lower leaf .