CAJ | 학술논문

目的探讨丝裂原活化蛋白激酶（MAPK）家族成员p38MAPK、细胞外信息调节蛋白激酶（ERK1/2）在非小细胞肺癌（NSCLC）中的表达及临床意义。方法选取43例NSCLC的肿瘤组织及其癌旁正常组织作为对照。采用蛋白免疫印迹法检测p38MAPK、ERK1/2的表达情况，分析其与临床特征（包括性别、年龄、肿块大小、淋巴结转移情况、分化程度、组织类型以及病理分期）的关系，及其表达程度与预后的关系。结果与配对的正常肺组织比较，phospho- p38MAPK、phospho- ERK1/2在NSCLC组织中活化上调（均P＜0.01），43例患者中37例phospho- p38MAPK的表达平均增长2.95倍，39例phospho- ERK1/2表达平均增长3.07倍，差异均有统计学意义（均P＜0.01）。ERK1/2的表达与各临床特征均无明显相关（均P＞0.05），而p38MAPK的活化与肿瘤大小以及年龄相关（均P＜0.01）。ERK1/2未活化组、中度活化组与高度活化组的生存曲线依次下移，p38MAPK高度活化组生存曲线明显降低。Cox比例风险回归模型分析显示，phospho- p38MAPK的高表达是预后不良的预测因素（P＜0.05）。结论 p38MAPK与ERK1/2的活化表达可能在NSCLC的发生/发展过程中具有重要意义，其中p38MAPK的活化程度可辅助作为非小细胞肺癌的预后评估因素。
목적탐토사렬원활화단백격매（MAPK）가족성원p38MAPK、세포외신식조절단백격매（ERK1/2）재비소세포폐암（NSCLC）중적표체급림상의의。방법선취43례NSCLC적종류조직급기암방정상조직작위대조。채용단백면역인적법검측p38MAPK、ERK1/2적표체정황，분석기여림상특정（포괄성별、년령、종괴대소、림파결전이정황、분화정도、조직류형이급병리분기）적관계，급기표체정도여예후적관계。결과여배대적정상폐조직비교，phospho- p38MAPK、phospho- ERK1/2재NSCLC조직중활화상조（균P＜0.01），43례환자중37례phospho- p38MAPK적표체평균증장2.95배，39례phospho- ERK1/2표체평균증장3.07배，차이균유통계학의의（균P＜0.01）。ERK1/2적표체여각림상특정균무명현상관（균P＞0.05），이p38MAPK적활화여종류대소이급년령상관（균P＜0.01）。ERK1/2미활화조、중도활화조여고도활화조적생존곡선의차하이，p38MAPK고도활화조생존곡선명현강저。Cox비례풍험회귀모형분석현시，phospho- p38MAPK적고표체시예후불량적예측인소（P＜0.05）。결론 p38MAPK여ERK1/2적활화표체가능재NSCLC적발생/발전과정중구유중요의의，기중p38MAPK적활화정도가보조작위비소세포폐암적예후평고인소。
Objective To examine the expression of phospho- p38MAPK, phospho- JNK, and phospho- ERK1/2 in non- smal celllung cancer (NSCLC) and its clinicopathological significance. Methods Forty- three specimens of primary non- smal celllung cancer and paired normal lung tissue were collected. The expressions of phospho- p38MAPK, phos-pho- ERK1/2 and phospho- JNK were detected with Western- blot analysis. The association between clinicopathological parame-ters and the expression of phospho- p38MAPK and phospho- ERK1/2 protein was analyzed. Results Compared with corre-sponding normal lung tissue, the expression of phospho- p38MAPK and phospho- ERK1/2 in NSCLC tissue were increased (P<0.01). In 37 cases, phospho- p38MAPK expression were increased by average 2.95 times (P<0.01), while in 39 cases phos-pho- ERK1/2 expression were increased by 3.07 times (P<0.01). The expression of ERK1/2 was not correlated with clinicpatho-logical parameters (P>0.05). The activation of p38MAPK was correlated with tumor size and ages of patients (both P<0.01). The expressions of ERK1/2 and p38MAPK were negatively correlated with survival. Multivariate analysis revealed that the expres-sion of phospho- p38MAPK was an independent prognostic indicator for overal survival of patients (P=0.046). Conclusion ERK1/2 and p38MAPK are activated in NSCLC, and the expression of phospho- p38MAPK can help to predict the prognosis of NSCLC.