CAJ | 학술논문

目的：探讨“miRNA海绵”在胃癌细胞系SGC7901上皮-间质转化（EMT）过程中的作用及机制。方法：将人工合成的ZEB23'UTR质粒及siZEB2采用脂质体Lipofectamine 2000转染SGC7901细胞。qRT-PCR检测转染后miR-200a/b/c的表达；Tran-swell侵袭实验、划痕实验和克隆形成实验检测细胞侵袭迁移增殖能力；Western Blot检测目的蛋白的表达。结果：与对照组相比， ZEB23'UTR转染组miR-200a/b/c的表达均下调，迁移、侵袭、增殖活性均增强；而转染siZEB2后miR-200a/b/c表达均升高，迁移、侵袭、增殖能力则下降。Western Blot显示ZEB23'UTR转染导致ZEB2表达升高，E-cadherin表达下降，而Vimentin表达上调；而转染siZEB2后，各项指标则表现出相反的表达趋势。结论：ZEB23'UTR可以通过调控miR-200a/b/c的表达调控胃癌细胞EMT过程，调节肿瘤的侵袭与转移。
목적：탐토“miRNA해면”재위암세포계SGC7901상피-간질전화（EMT）과정중적작용급궤제。방법：장인공합성적ZEB23'UTR질립급siZEB2채용지질체Lipofectamine 2000전염SGC7901세포。qRT-PCR검측전염후miR-200a/b/c적표체；Tran-swell침습실험、화흔실험화극륭형성실험검측세포침습천이증식능력；Western Blot검측목적단백적표체。결과：여대조조상비， ZEB23'UTR전염조miR-200a/b/c적표체균하조，천이、침습、증식활성균증강；이전염siZEB2후miR-200a/b/c표체균승고，천이、침습、증식능력칙하강。Western Blot현시ZEB23'UTR전염도치ZEB2표체승고，E-cadherin표체하강，이Vimentin표체상조；이전염siZEB2후，각항지표칙표현출상반적표체추세。결론：ZEB23'UTR가이통과조공miR-200a/b/c적표체조공위암세포EMT과정，조절종류적침습여전이。
Objective:To explore the effect and mechanism of miRNA sponge on the epithelial-mesenchymal transition (EMT) of gastric carcinoma cell lines SGC7901. Methods:Synthetic ZEB2 3'UTR plasmid and siRNA targeting ZEB2 were transfected into the SGC7901 cell line by Lipofectamine 2000. Real-time quantitative polymerase chain reaction was performed to evaluate the expres-sion levels of miR-200a/b/c. Finally, the migratory, invasive, and proliferative activities of the gastric carcinoma cells in vitro were ana-lyzed by the scratch test, the Transwell cell invasion, and the cell cloning assay. The expression of the target protein was detected by Western blot. Results:Compared with the control group, the expressions of miR-200a/b/c significantly decreased, and their migration, invasion, and proliferation capabilities were considerably higher after they were transfected with ZEB2 3'UTR. Although the expres-sions of miR-200a/b/c significantly increased, the migratory, invasive, and proliferative activities of SGC7901 cells also degraded after they were transfected with siRNA targeting ZEB2. The expression of ZEB2 increased, and that of E-cadherin decreased at the protein level after they were transfected with ZEB2 3'UTR. The protein expression of Vimentin in SGC7901 cells significantly increased. The indicators show the opposite trend when cells were transfected with siZEB2, and the differences between the control and mutation groups were insignificant. Conclusion:ZEB2 3'UTR can regulate EMT course by regulating the miR-200a/b/c expression in gastric car-cinoma, consequently regulating the invasion and migration of carcinoma cells.