CAJ | 학술논문

目的：明确miR-30b对结直肠癌细胞侵袭和迁移潜能的影响。方法：RT-qPCR检测20对配对结直肠癌组织标本中miR-30b的表达；Transwell和划痕实验检测过表达和抑制miR-30b后结直肠癌细胞侵袭和迁移潜能的改变；应用生物信息学方法预测miR-30b的作用靶点；Western Blot及双荧光素酶报告基因实验验证过表达和抑制miR-30b后靶点Snail和EMT（epithelial mesenchymal transition）标志物的表达情况。结果：癌组织中miR-30b表达水平明显低于正常肠黏膜组织；Transwell及划痕实验结果显示过表达miR-30b后结直肠癌细胞的侵袭迁移能力降低，抑制miR-30b后侵袭迁移能力增强；生物信息学预测结果显示miR-30b能够作用于Snail 3'-UTR，双荧光素酶检测结果进一步证实miR-30b能够作用于Snail 3'-UTR；Western Blot结果显示过表达miR-30b后Snail的表达下降，EMT标志物Vimentin表达下降和E-cadherin表达升高，而抑制miR-30b后结果相反。结论：miR-30b在结直肠癌中表达水平下降，并通过靶向Snail调节结直肠癌细胞的侵袭和迁移。
목적：명학miR-30b대결직장암세포침습화천이잠능적영향。방법：RT-qPCR검측20대배대결직장암조직표본중miR-30b적표체；Transwell화화흔실험검측과표체화억제miR-30b후결직장암세포침습화천이잠능적개변；응용생물신식학방법예측miR-30b적작용파점；Western Blot급쌍형광소매보고기인실험험증과표체화억제miR-30b후파점Snail화EMT（epithelial mesenchymal transition）표지물적표체정황。결과：암조직중miR-30b표체수평명현저우정상장점막조직；Transwell급화흔실험결과현시과표체miR-30b후결직장암세포적침습천이능력강저，억제miR-30b후침습천이능력증강；생물신식학예측결과현시miR-30b능구작용우Snail 3'-UTR，쌍형광소매검측결과진일보증실miR-30b능구작용우Snail 3'-UTR；Western Blot결과현시과표체miR-30b후Snail적표체하강，EMT표지물Vimentin표체하강화E-cadherin표체승고，이억제miR-30b후결과상반。결론：miR-30b재결직장암중표체수평하강，병통과파향Snail조절결직장암세포적침습화천이。
Objective:To determine the function of miR-30b in the metastasis of colorectal cancer cells. Methods:RT-qPCR was performed to test miR-30b expression in 20 fresh primary colorectal cancer tissues and their corresponding adjacent tissues. Transwell and wound healing assays were performed to test the invasion and migration of colorectal cancer cells after miR-30b overexpression or inhibition. Bioinformatics assay was performed to predict miR-30b targets. Western Blot and Dual Luciferase reporter assay were per-formed to test the expressions of Snail and downstream target genes in colorectal cancer cells. Results:The results reveal that miR-30b expression decreased in cancer tissues compared with normal tissues. Transwell and wound healing assays reveal that miR-30b overex-pression inhibited cell invasion and migration, whereas miR-30b inhibition promoted the invasion and migration of colorectal cancer cells. Bioinformatics analyses reveal that miR-30b targets the 3'-UTR of Snail. Dual Luciferase reporter assay confirms that miR-30b af-fects the 3'-UTR of Snail. Western Blot analyses show that Snail and Vimentin expressions were significantly downregulated, whereas E-cadherin expression obviously increased after miR-30b overexpression. However, Snail and Vimentin expressions increased, but E-cadherin expression decreased after miR-30b inhibition. Conclusion:The miR-30b gene is downregulated in colorectal cancer tis-sues. The miR-30b protein may be important in the regulation of cell invasion and migration by targeting Snail in colorectal cancer cells.