CAJ | 학술논문

目的：采用高效液相色谱法(HPLC法)测定合剂(前胡桑菊合剂、五柑合剂、胃肠合剂、佛耳合剂)中苯甲酸的含量。方法用乙酸乙酯提取合剂中的苯甲酸,提取液浓缩后采用HPLC法进行测定。选择Thermo Syncronis C18(250 mm×4.6 mm,5μm),以甲醇-磷酸二氢钾(0.05 mol/L)(45：55)为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为226 nm。结果苯甲酸回归方程Y=5835283.5514 X-23440.3860(R=0.9999),在0.020~0.1018 mg/ml范围内线性关系良好；前胡桑菊合剂、五柑合剂、胃肠合剂、佛耳合剂平均回收率分别为99.79%、99.37%、99.34%、99.92%；RSD分别为0.35%、0.10%、0.76%、0.36%(n=3)。结论测定方法简便、准确、专属性强、重现性良好。
목적：채용고효액상색보법(HPLC법)측정합제(전호상국합제、오감합제、위장합제、불이합제)중분갑산적함량。방법용을산을지제취합제중적분갑산,제취액농축후채용HPLC법진행측정。선택Thermo Syncronis C18(250 mm×4.6 mm,5μm),이갑순-린산이경갑(0.05 mol/L)(45：55)위류동상,류속위1.0 ml/min,주온위30℃,검측파장위226 nm。결과분갑산회귀방정Y=5835283.5514 X-23440.3860(R=0.9999),재0.020~0.1018 mg/ml범위내선성관계량호；전호상국합제、오감합제、위장합제、불이합제평균회수솔분별위99.79%、99.37%、99.34%、99.92%；RSD분별위0.35%、0.10%、0.76%、0.36%(n=3)。결론측정방법간편、준학、전속성강、중현성량호。
Objective To establish a high performance liquid chromatography(HPLC) method for the determination of(Qianhu Sangju mixture, Wugan mixture, Weichang mixture, Foer mixture) benzoic acid. Methods The extraction mixture of benzoic acid with ethyl acetate, the concentrated extract was determined by HPLC method. Select the thermo syncronis C-18(250 mm×4.6 mm, 5 μm), with methanol and potassium dihydrogen phosphate(0.05 mol/L) (45:55) as mobile phase, flow rate of 1.0 ml/min, column temperature was 30℃ , the detection wavelength was 226 nm. Results Benzoic acid regression equation was Y=5835283.5514 X-23440.3860(R=0.9999), in 0.020~0.1018 mg/ml the linear relationship was good; Qinhu Sangju mixture, Wugan mixture, Weichang mixture, Foer mixture the average recovery rate was 99.79%, 99.37%, 99.34%, 99.92%;RSD=0.35%, 0.10%, 0.76%, 0.36%(n=3). Conclusion This method is simple and accurate, with a strong specificity, so it can be used as preparation for the quality control.