CAJ | 학술논문

目的：了解宁夏地区食品中单核细胞增生李斯特菌（LM）污染情况，为食源性LM污染的预防控制与干预提供依据。方法2006～2012年设立5个监测点（银川市、石嘴山市、吴忠市、中卫市、固原市）进行采样，共监测样本4492份，6大类食品。按照GB 4789.30-2010《食品安全国家标准食品微生物学检验单粒细胞增生李斯特氏菌检验》进行样品中菌株的分离鉴定。结果从4492份样品中共分离出 LM 40株，总检出率为0.89%，2006年检出率最高，为3.42%，2012年检出率最低，为0.31%，各年度食品中LM检出率比较，差异有统计学意义（χ2=38.171，P=0.000）。生肉阳性率最高（2.71%），其次为熟肉制品（1.94%）、动物水产品（1.54%）和速冻米面制品（0.73%），各类食品LM检出率比较，差异有统计学意义（χ2=50.207，P=0.000）。从病原菌检出率看，银川市食品LM污染情况较严重，其他依次为中卫市、石嘴山市、吴忠市、固原市，各地区LM检出率比较，差异有统计学意义（χ2=21.434，P=0.000）。结论宁夏回族自治区部分食品中存在不同程度的LM污染，应针对LM污染严重的生肉类、熟肉制品及水产品进行重点监督和监测。
목적：료해저하지구식품중단핵세포증생리사특균（LM）오염정황，위식원성LM오염적예방공제여간예제공의거。방법2006～2012년설립5개감측점（은천시、석취산시、오충시、중위시、고원시）진행채양，공감측양본4492빈，6대류식품。안조GB 4789.30-2010《식품안전국가표준식품미생물학검험단립세포증생리사특씨균검험》진행양품중균주적분리감정。결과종4492빈양품중공분리출 LM 40주，총검출솔위0.89%，2006년검출솔최고，위3.42%，2012년검출솔최저，위0.31%，각년도식품중LM검출솔비교，차이유통계학의의（χ2=38.171，P=0.000）。생육양성솔최고（2.71%），기차위숙육제품（1.94%）、동물수산품（1.54%）화속동미면제품（0.73%），각류식품LM검출솔비교，차이유통계학의의（χ2=50.207，P=0.000）。종병원균검출솔간，은천시식품LM오염정황교엄중，기타의차위중위시、석취산시、오충시、고원시，각지구LM검출솔비교，차이유통계학의의（χ2=21.434，P=0.000）。결론저하회족자치구부분식품중존재불동정도적LM오염，응침대LM오염엄중적생육류、숙육제품급수산품진행중점감독화감측。
Objective To understand the contamination condition of Listeria monocytogenes (LM) in foods of Ningxia,in order to provide a scientific basis for control and prevention of foodborne LM contamination. Methods A total of 4 492 samples were monitored in 5 monitoring points(Yinchuan city,Shizuishan city,Wuzhong city,Zhongwei city,Guyuan city) set from 2006 to 2012,including 6 kinds of food. National standards method (GB4789.30-2010) and National Foodborne Disease Surveillance Network Monitoring Program were used to isolate and identify LM from collected samples. Results Totally 40 strains of LM were detected from 4 492 samples with the total detection rate of 0.89%,the year with the highest detection rate was 2006 (3.42%),and the lowest was 2012 (0.31%) the comparison of LM detection rate among the seven years had statistically significant difference (χ2=38.171,P=0.000). The raw meat had the highest positive rate(2.71%),followed by cooked meat products(1.54%),aquatic animal products (1.54%) and quick-freeze rice/flours products (0.73%),and the detection rate of LM among different products had statistically significant difference (χ2=50 . 207 , P=0 . 000 ) . From the detection rate of pathogenic bacteria ,the most serious LM contamination appeared in Yinchuan city,followed by Zhongwei city,Shizuishan city,Wuzhong city and Guyuan city,and the comparison of LM detection rate among different regions had statistically significant difference (χ2=21.434,P=0.001). Conclusion Some food is polluted by LM in varying degrees in Ninxiang Hui Autonomous regions ,supervision and monitoring should em-phasized on raw meat,cooked meat products and aquatic products due to serious pollution of LM.