CAJ | 학술논문

目的：观察不同培养基对人脐带间充质干细胞(hUC-MSCs)生物学特性的影响,优化培养方法。方法采用贴壁法从正常足月胎儿脐带中分离出间充质干细胞,细胞贴壁后利用DMEM/F12, Mesen PRORSTM Medium这两种培养体系进行体外扩增,绘制生长曲线,流式细胞仪检测其表面标志。结果从人脐带中分离出的间充质干细胞为梭形,呈平行排列生长或漩涡状生长；细胞表达CD29、CD44,不表达CD34、CD45和HLA-DR。结论加入Mesen PRORSTM Medium培养的细胞增殖速度快,更适合于脐带间充质干细胞的体外扩增。
목적：관찰불동배양기대인제대간충질간세포(hUC-MSCs)생물학특성적영향,우화배양방법。방법채용첩벽법종정상족월태인제대중분리출간충질간세포,세포첩벽후이용DMEM/F12, Mesen PRORSTM Medium저량충배양체계진행체외확증,회제생장곡선,류식세포의검측기표면표지。결과종인제대중분리출적간충질간세포위사형,정평행배렬생장혹선와상생장；세포표체CD29、CD44,불표체CD34、CD45화HLA-DR。결론가입Mesen PRORSTM Medium배양적세포증식속도쾌,경괄합우제대간충질간세포적체외확증。
Objective To investigate the differences of biological characteristics of human umbilical cord mesenchymal stem cells(hUC-MSCs) in different mediums, and to optimize the purify methods. Methods Mesenchymal stem cells were separated from healthy full-termed delivery fetus using tissue-adherent culture method. These cells were cultured and amplified in DMEM/F12 and Mesen PRORSTM Medium in vitro. The growth curve was drawn.Their surface markers were detected by flow cytometry.Results Mesenchymal stem cells derived from human umbilical cord were spindle-shaped adherent cells.They have strong proliferation ability. The results of growth curve showed that compared with two medium, hUC-MSCs in Mesen PRORSTM Medium grew quickly at every time point. They were strongly positive expression for CD44 and CD29, but negative expression for CD34, CD45 and HLA-DR.Conclusion Cell doubling time in the Mesen PRORSTM Medium was shorter than in DMEM/F12.