CAJ | 학술논문

目的：研究人脐带血间充质干细胞向胰岛素分泌细胞分化的潜能及其移植后对糖尿病大鼠的治疗效果。方法体外分离培养HUCB-MSCs,在胰岛细胞培养条件下经药物定向诱导其分化；免疫组化对诱导细胞进行胰岛β细胞标记鉴定；双硫腙染色鉴定锌离子表达及检测胰岛样细胞的移植效果。结果 HUCB-MSCs经诱导后,免疫细胞化学染色显示表达人胰岛素；双硫腙染色呈棕红色；移植后2周,胰岛样细胞组血糖浓度明显降低。结论 HUCB-MSCs在体外诱导培养条件下,具有向胰岛素分泌细胞分化的潜能,这种细胞可能为Ⅰ型糖尿病提供一条新的治疗途径。
목적：연구인제대혈간충질간세포향이도소분비세포분화적잠능급기이식후대당뇨병대서적치료효과。방법체외분리배양HUCB-MSCs,재이도세포배양조건하경약물정향유도기분화；면역조화대유도세포진행이도β세포표기감정；쌍류종염색감정자리자표체급검측이도양세포적이식효과。결과 HUCB-MSCs경유도후,면역세포화학염색현시표체인이도소；쌍류종염색정종홍색；이식후2주,이도양세포조혈당농도명현강저。결론 HUCB-MSCs재체외유도배양조건하,구유향이도소분비세포분화적잠능,저충세포가능위Ⅰ형당뇨병제공일조신적치료도경。
Objective To explore the possibility of inducing the differentiation of human umbilical cord blood mesenchymal stem cells(HUCB-MSCs) into insulin secreting cells in vitro and the therapeutic efficacy on diabetic rats following transplantation.Methods We isolated and cultured HUCB-MSCs, and induced HUCB-MSCs to differentiate into insulin secreting cells in the islet cell culture condition. HUCB-MSCs of induction were detected by immunocytochemical Methods. Zinchydronium was detected by Dithizon staining. Transplantation efficiency of islet-like cells was detected.Results Under induction, immunocytochemical examination showed that the expression of human insulin was positive. Dithizon stain showed that the cytoplasm of HUCB-MSCs was stained in brownish red color after induction. At 2 weeks following transplantation, blood glucose concentration in the islet-like cell group significantly reduced.Conclusion HUCB-MSCs can be differentiated into insulin secreting cells in vitro. HUCB-MSCs have the potential to become an excellent candidate in β cell replacement therapy of typeⅠdiabetes.