CAJ | 학술논문

目的：探讨脑源性神经营养因子(BDNF)基因功能性多态(rs6265)与散发性阿尔茨海默病(SAD)发病的相关性。方法选取58例散发性阿尔茨海默患者(SAD组)与52例健康老年人(对照组),用聚合酶链反应-限制性片段长度多态性技术,对BDNF基因rs6265,进行基因型检测,同时利用酶联免疫吸附技术对两组患者的血清BDNF水平进行检测。结果在<60岁的人群中,等位基因(χ2=6.0595, P=0.013)及基因型(χ2=6.0826,P=0.0478)在两组人群中的分布差异有统计学意义,并且SAD的AA基因型患者的血清BDNF水平最低[(14.32±4.21)ng/ml, F=7.2545, P=0.0016]。结论 BDNF基因功能性多态rs6265与SAD发病相关,并且影响其血清BDNF的表达。
목적：탐토뇌원성신경영양인자(BDNF)기인공능성다태(rs6265)여산발성아이자해묵병(SAD)발병적상관성。방법선취58례산발성아이자해묵환자(SAD조)여52례건강노년인(대조조),용취합매련반응-한제성편단장도다태성기술,대BDNF기인rs6265,진행기인형검측,동시이용매련면역흡부기술대량조환자적혈청BDNF수평진행검측。결과재<60세적인군중,등위기인(χ2=6.0595, P=0.013)급기인형(χ2=6.0826,P=0.0478)재량조인군중적분포차이유통계학의의,병차SAD적AA기인형환자적혈청BDNF수평최저[(14.32±4.21)ng/ml, F=7.2545, P=0.0016]。결론 BDNF기인공능성다태rs6265여SAD발병상관,병차영향기혈청BDNF적표체。
Objective To explore the relationship between functional polymorphism of BDNF(brain-derived neurotrophic factor) rs6265 and sporadic Alzheimer’s disease(SAD). Methods Selecting 58 cases of SAD(SAD group) and 52 health old person(control group) as research subjects. Then the genotyped was detected via polymerase chain reaction-restriction fragment length polymorphism and serum levels of BDNF detected by Enzyme-linked Immunosorbent Assay in all cases. Results There was significantly difference in the distributions of alleles(χ2=6.0595,P=0.013) and genotypes(χ2=6.0826,P=0.0478) between the case of SAD group and control group ,and the serum levels of BDNF were lowest for case of AA genotypes in SAD[(14.32±4.21) ng/ml, F=7.2545, P=0.0016]. Conclusion The functional polymorphism of BDNF gene(rs6265) correlated with pathogenesis of SAD and affects the expression of BDNF in serum.