CAJ | 학술논문

以筒柱藻B200为试验材料，采用室内一次性充气培养法和叶绿素荧光分析技术，研究了硝酸钠、氯化铵、尿素和磷酸二氢钠、甘油磷酸钠、三磷酸腺苷二钠对其叶绿素荧光特性和生长的影响。试验结果表明，第1～4d，尿素组光系统Ⅱ的最大光能转化效率最大，第7～9d，硝酸钠组的该参数最大；第3～9d，硝酸钠组的最大光合作用速率和光化学淬灭最大，尿素组的上述两个参数在整个培养周期内最小；第1～7d，硝酸钠组快速光曲线的初始斜率最大；第4～9d，硝酸钠组的半饱和光照度最大。试验结束时，硝酸钠组的最终细胞密度和干质量均显著高于其他两个处理组；叶绿素含量氯化铵组最小，硝酸钠组和尿素组较高，但两组之间差异不显著。不同磷源对筒柱藻的叶绿素荧光参数和生长也有显著影响，第5～9d，磷酸二氢钠组光系统Ⅱ的最大光能转化效率最大，三磷酸腺苷二钠组的该参数最小；第3～9d，磷酸二氢钠组的光化学淬灭和快速光曲线的初始斜率均显著高于其他两个处理组；第6～9d，磷酸二氢钠组的最大光合作用速率和半饱和光照度最大，三磷酸腺苷二钠组的上述两个参数最小。试验结束时，磷酸二氢钠组的最终细胞密度、干质量和叶绿素含量均显著高于其他两个处理组。
이통주조B200위시험재료，채용실내일차성충기배양법화협록소형광분석기술，연구료초산납、록화안、뇨소화린산이경납、감유린산납、삼린산선감이납대기협록소형광특성화생장적영향。시험결과표명，제1～4d，뇨소조광계통Ⅱ적최대광능전화효솔최대，제7～9d，초산납조적해삼수최대；제3～9d，초산납조적최대광합작용속솔화광화학쉬멸최대，뇨소조적상술량개삼수재정개배양주기내최소；제1～7d，초산납조쾌속광곡선적초시사솔최대；제4～9d，초산납조적반포화광조도최대。시험결속시，초산납조적최종세포밀도화간질량균현저고우기타량개처리조；협록소함량록화안조최소，초산납조화뇨소조교고，단량조지간차이불현저。불동린원대통주조적협록소형광삼수화생장야유현저영향，제5～9d，린산이경납조광계통Ⅱ적최대광능전화효솔최대，삼린산선감이납조적해삼수최소；제3～9d，린산이경납조적광화학쉬멸화쾌속광곡선적초시사솔균현저고우기타량개처리조；제6～9d，린산이경납조적최대광합작용속솔화반포화광조도최대，삼린산선감이납조적상술량개삼수최소。시험결속시，린산이경납조적최종세포밀도、간질량화협록소함량균현저고우기타량개처리조。
The effects of 3 nitrogen sources (sodium nitrate , ammonium chloride and urea ) and 3 phosphorus sources (sodium dihydrogen phosphate , sodium glycerophosphate and disodium adenosine triphosphate) on chlorophyll fluorescence characteristics and growth in green alga Cylindrotheca sp .B200 were studied by aerated indoor batch culture method and chlorophyll fluorescence analysis .The results showed that the maximal values of Fv/Fm (the maximum photochemical efficiency of PS Ⅱ) were observed in urea treatment from the 1st day to the 4th day and in sodium nitrate treatment from the 7th day to the 9th day ,respectively .For the parameters Pm (the maximum efficiency of photosynthesis ) and qP (the photochemical quenching) ,the maximal values were recorded in sodium nitrate treatment from the 3rd day to the 9th day and the minimal values were recorded in urea treatment during the entire experimental period .The maximal values of α (the initial slope of the rapid light curve) were detected in sodium nitrate treatment from the 1st day to the 7th day ,while those of Ik (the half saturation of light intensity ) were recorded in sodium nitrate treatment from the 4th day to the 9th day .At the end of experiment ,there were higher final cell density and dry weight in sodium nitrate treatment than those in other treatments .The minimal chlorophyll contents were shown in ammonium chloride treatment while the higher values were found in sodium nitrate and urea treatments ,without significant difference in the two treatments .For phosphorus sources , the maximal values of Fv/Fm were observed in sodium dihydrogen phosphate treatment w hile the minimal values in disodium adenosine triphosphate treatment from the 5th day to the 9th day .The parameters qP and α were found significantly higher under sodium dihydrogen phosphate treatment than those in other treatments from the 3rd day to the 9th day .The maximal and minimal Pm and Ik values were recorded in sodium dihydrogen phosphate and disodium adenosine triphosphate treatments from the 6th day to the 9thday ,respectively .At the end of experiment ,the final cell density ,dry weight and chlorophyll content were significantly higher in the sodium dihydrogen phosphate treatment than those in other two treatments .