中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2014年
17期
8-9
,共2页
血府逐瘀口服液%苦杏仁苷%高效液相色谱法%蒸发光散射检测器%含量测定
血府逐瘀口服液%苦杏仁苷%高效液相色譜法%蒸髮光散射檢測器%含量測定
혈부축어구복액%고행인감%고효액상색보법%증발광산사검측기%함량측정
Xue Fu Zhu Yu oral liquid%Amygdalin%High performance liquid chromatography%Evaporative light-scattering detector%Content determination
目的：建立测定血府逐瘀口服液中苦杏仁苷的含量的方法。方法：采用高效液相色谱-蒸发光散射检测器法。色谱柱为十八烷基硅烷键合硅胶为填充剂，流动相为甲醇-水(25：75)，流速控制在1ml/分，柱温控制在30℃；蒸发光散射检测器的漂移管温度控制105℃，空气或氮气作为其中的雾化气体，其流速控制在3.0L/分。结果：苦杏仁苷的进样量0.856～4.28μg，其峰面积的自然对数与进样量的自然对数具有良好的线性关系(r=0.9995)，平均回收率99.06%，RSD=1.39(n=6)。结论：该方法灵敏、准确，可用于血府逐瘀口服液中苦杏仁苷的含量测定。
目的：建立測定血府逐瘀口服液中苦杏仁苷的含量的方法。方法：採用高效液相色譜-蒸髮光散射檢測器法。色譜柱為十八烷基硅烷鍵閤硅膠為填充劑，流動相為甲醇-水(25：75)，流速控製在1ml/分，柱溫控製在30℃；蒸髮光散射檢測器的漂移管溫度控製105℃，空氣或氮氣作為其中的霧化氣體，其流速控製在3.0L/分。結果：苦杏仁苷的進樣量0.856～4.28μg，其峰麵積的自然對數與進樣量的自然對數具有良好的線性關繫(r=0.9995)，平均迴收率99.06%，RSD=1.39(n=6)。結論：該方法靈敏、準確，可用于血府逐瘀口服液中苦杏仁苷的含量測定。
목적：건립측정혈부축어구복액중고행인감적함량적방법。방법：채용고효액상색보-증발광산사검측기법。색보주위십팔완기규완건합규효위전충제，류동상위갑순-수(25：75)，류속공제재1ml/분，주온공제재30℃；증발광산사검측기적표이관온도공제105℃，공기혹담기작위기중적무화기체，기류속공제재3.0L/분。결과：고행인감적진양량0.856～4.28μg，기봉면적적자연대수여진양량적자연대수구유량호적선성관계(r=0.9995)，평균회수솔99.06%，RSD=1.39(n=6)。결론：해방법령민、준학，가용우혈부축어구복액중고행인감적함량측정。
Objective:To develop a method for the content determination of amygdalin in Xue Fu Zhu Yu oral liquid.Methods:High performance liquid chromatography-evaporative light-scattering detector method was used.The chromatographic column was octadecylsilane chemically bonded silica as the filler,the moving phase was methanol-water(25:75),the flow velocity was 1ml one minute,the column temperature was 30℃.The drift tube temperature of evaporative light-scattering detector was 105℃,air or nitrogen was as the atomizing gas,its flow velocity was 3.0L one minute.Results:The incoming sample amount of amygdalin was 0.856~4.28 μ g,the natural logarithm of peak area and the natural logarithm of incoming sample amount had good linear relationship(r=0.9995),the average recovery was 99.06% ,RSD=1.39(n=6).Conclusion:This method is sensitive,accurate,can be used for the content determination of amygdalin in Xue Fu Zhu Yu oral liquid.
