医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2014年
17期
135-136
,共2页
含量测定%复方醋酸泼尼松龙滴鼻液%RP-HPLC
含量測定%複方醋痠潑尼鬆龍滴鼻液%RP-HPLC
함량측정%복방작산발니송룡적비액%RP-HPLC
Determination of content%Prednisolone acetate nose drops%RP-HPLC
目的：建立高效液相色谱法测定复方醋酸泼尼松龙滴鼻液中醋酸泼尼松龙的含量的方法。方法采用Phenomenex Gemini C18（250mm×4.6mm，5μm）色谱柱，以乙腈-水（35：65）为流动相，流速为1.0mL·min-1，检测波长为246nm。结果醋酸泼尼松龙在在0.009642~0.2411mg·ml-1范围内浓度与峰面积呈良好线性关系（r2=1），平均回收率为101.01%，RSD为0.7%（n=9）。结论本方法简便、快速、结果准确性、重现性好。
目的：建立高效液相色譜法測定複方醋痠潑尼鬆龍滴鼻液中醋痠潑尼鬆龍的含量的方法。方法採用Phenomenex Gemini C18（250mm×4.6mm，5μm）色譜柱，以乙腈-水（35：65）為流動相，流速為1.0mL·min-1，檢測波長為246nm。結果醋痠潑尼鬆龍在在0.009642~0.2411mg·ml-1範圍內濃度與峰麵積呈良好線性關繫（r2=1），平均迴收率為101.01%，RSD為0.7%（n=9）。結論本方法簡便、快速、結果準確性、重現性好。
목적：건립고효액상색보법측정복방작산발니송룡적비액중작산발니송룡적함량적방법。방법채용Phenomenex Gemini C18（250mm×4.6mm，5μm）색보주，이을정-수（35：65）위류동상，류속위1.0mL·min-1，검측파장위246nm。결과작산발니송룡재재0.009642~0.2411mg·ml-1범위내농도여봉면적정량호선성관계（r2=1），평균회수솔위101.01%，RSD위0.7%（n=9）。결론본방법간편、쾌속、결과준학성、중현성호。
Objective To establisha method for determination content of prednisolone acetate in the co-prednisolone acetate nose drops by RP-HPLC.Methods Phenominix Gemini C18(250mm×4.6mm,5μm)column was used and acetonitrile -water(35:65)was used as mobile phase, the flow rate was 1.0mLomin-1,the detecting wave-length was at 246nm.Results There was a good linear relationship between the concentration of prednisolone acetate and peak area within a range of 0.009642~0.2411mg·ml-1(r2=1).The average recover was 101.01%,with RSD=0.7%(n=9).Conclusion Themethod is highly repeatable and accurate.
