CAJ | 학술논문

目的：探讨兔肺内肿瘤射频消融（ RFA）后残存肿瘤细胞增殖和新生血管的变化。方法采用组织块悬液注射法建立兔VX2肿瘤肺内种植模型，64只新西兰白兔随机分为对照组（n＝10）和RFA组（n＝54）。以控制电极展开范围、输出功率和治疗时间的方法，造成RFA后肿瘤残存。采用免疫组化法计算不同时间点残存肿瘤细胞Ki-67标记指数（Ki-67LI）和微血管密度（MVD）。结果对照组Ki-67LI 为45.3％±2.1％。 RFA组Ki-67LI在第1、3、5天分别为56.4％±3.4％、60.1％±4.1％和59.8％±2.4％，显著高于对照组（均 P ＜0.05）；在第7、9、14和21天分别为45.4％±2.0％、46.2％±3.4％、45.1％±4.4％和47.8％±3.9％，与对照组比较差异无统计学意义。对照组MVD为（28.9±2.9）条。 RFA组MVD在第3、5、7天分别为（36.8±2.6）、（55.6±4.8）和（51.5±2.8）条，显著高于对照组（均P＜0.05）；在第1、9、14、21天分别为（27.0±2.8）、（29.2±3.2）、（30.0±2.8）和（28.8±3.1）条，与对照组比较差异无统计学意义。结论 RFA后肺内残存肿瘤出现细胞增殖和新生血管一过性升高现象。
목적：탐토토폐내종류사빈소융（ RFA）후잔존종류세포증식화신생혈관적변화。방법채용조직괴현액주사법건립토VX2종류폐내충식모형，64지신서란백토수궤분위대조조（n＝10）화RFA조（n＝54）。이공제전겁전개범위、수출공솔화치료시간적방법，조성RFA후종류잔존。채용면역조화법계산불동시간점잔존종류세포Ki-67표기지수（Ki-67LI）화미혈관밀도（MVD）。결과대조조Ki-67LI 위45.3％±2.1％。 RFA조Ki-67LI재제1、3、5천분별위56.4％±3.4％、60.1％±4.1％화59.8％±2.4％，현저고우대조조（균 P ＜0.05）；재제7、9、14화21천분별위45.4％±2.0％、46.2％±3.4％、45.1％±4.4％화47.8％±3.9％，여대조조비교차이무통계학의의。대조조MVD위（28.9±2.9）조。 RFA조MVD재제3、5、7천분별위（36.8±2.6）、（55.6±4.8）화（51.5±2.8）조，현저고우대조조（균P＜0.05）；재제1、9、14、21천분별위（27.0±2.8）、（29.2±3.2）、（30.0±2.8）화（28.8±3.1）조，여대조조비교차이무통계학의의。결론 RFA후폐내잔존종류출현세포증식화신생혈관일과성승고현상。
Objective To observe the changes of proliferation and angiogenesis of residual tumor in rabbit lung after radiofrequency ablation ( RFA).Methods The model of VX2 tumor in rabbit lung was established by injection of tissue block suspension.64 New Zealand White rabbits bearing VX 2 tumor were assigned randomly to the control group (n=10) and the RFA group (n=48).During the RFA procedure, residual tumors were achieved by controlling the range of electrode expanding , output power and treatment time.At several points of time, Ki-67 labeling index (Ki-67LI) and microvessel density (MVD) of the residual tumors were calculated by immunohistochemical detection.Results Ki-67LI of the control group was 45.3%±2.1%.Ki-67LI of the RFA group at the first, 3 and 5 day were 56.4%±3.4%, 60.1%± 4.1%and 59.8%±2.4%respectively , significantly higher than that of the control group;however , at the seventh, 9, 14 and 21 day, they were 45.4%±2.0%, 46.2%±3.4%, 45.1%±4.4% and 47.8%± 3.9%respectively , no significant difference compared with the control group.The control group MVD was 28.9 ±2.9.MVD of the RFA group at third, 5 and 7 day were 36.8 ±2.6, 55.6 ±4.8 and 51.5 ±2.8 respectively, significantly higher than that of the control group;however, at the first, 9, 14 and 21 day were 27 ±2.8, 29.2 ±3.2, 30 ±2.8 and 28.8 ±3.1 respectively, no significant difference compared with the control group.Conclusions The proliferation and angiogenesis of pulmonary residual tumor exhibit a transient increase phenomenon after RFA.