CAJ | 학술논문

目的：探究阿尔茨海默病相关神经丝蛋白（AD7C-NTP）在阿尔茨海默病源性轻度认知功能损害患者诊断的临床价值。方法选取2012年12月至2013年9月就诊于广西医科大学第一附属医院神经内科病房及门诊的患者及正常人，采用酶联免疫吸附测定方法（ELISA），检测88例老年人（ MCI组52例，健康对照组36例）的脑脊液、血液、尿液中 AD7C-NTP 含量，所有数据均经SPSS18.0统计软件进行统计学分析。结果脑脊液AD7C-NTP含量：MCI组（637±191） ng/L，健康对照组（372±98）ng/L；血液AD7C-NTP含量：MCI组（499±139）ng/L，对照组（271±105）ng/L；尿液AD7C-NTP含量：MCI组（343±99）ng/L，健康对照组（195±76）ng/L，通过ROC曲线分析：当脑脊液AD7C-NTP含量为436.97 ng/L时，对应的灵敏度和特异度之和最大，分别为86.7％和85.0％；当血液AD7C-NTP含量为367.06 ng/L时，对应的灵敏度和特异度之和最大，分别为76.7％和85.0％；当尿液AD7C-NTP含量为268.57 ng/L时，对应的灵敏度和特异度之和最大，分别为83.3％和90.0％。结论 AD7C-NTP在MCI患者脑脊液、血液及尿液中的含量均升高，且和健康对照组差异显著；脑脊液及尿液AD7C-NTP含量诊断MCI的敏感度和特异度较高，且较接近；AD7C-NTP对MCI的临床诊断价值值得推广。
목적：탐구아이자해묵병상관신경사단백（AD7C-NTP）재아이자해묵병원성경도인지공능손해환자진단적림상개치。방법선취2012년12월지2013년9월취진우엄서의과대학제일부속의원신경내과병방급문진적환자급정상인，채용매련면역흡부측정방법（ELISA），검측88례노년인（ MCI조52례，건강대조조36례）적뇌척액、혈액、뇨액중 AD7C-NTP 함량，소유수거균경SPSS18.0통계연건진행통계학분석。결과뇌척액AD7C-NTP함량：MCI조（637±191） ng/L，건강대조조（372±98）ng/L；혈액AD7C-NTP함량：MCI조（499±139）ng/L，대조조（271±105）ng/L；뇨액AD7C-NTP함량：MCI조（343±99）ng/L，건강대조조（195±76）ng/L，통과ROC곡선분석：당뇌척액AD7C-NTP함량위436.97 ng/L시，대응적령민도화특이도지화최대，분별위86.7％화85.0％；당혈액AD7C-NTP함량위367.06 ng/L시，대응적령민도화특이도지화최대，분별위76.7％화85.0％；당뇨액AD7C-NTP함량위268.57 ng/L시，대응적령민도화특이도지화최대，분별위83.3％화90.0％。결론 AD7C-NTP재MCI환자뇌척액、혈액급뇨액중적함량균승고，차화건강대조조차이현저；뇌척액급뇨액AD7C-NTP함량진단MCI적민감도화특이도교고，차교접근；AD7C-NTP대MCI적림상진단개치치득추엄。
Objective To explore the diagnostic value of Alzheimer-associated neuronal thread protein ( AD7C-NTP ) in the assessment of patients with mild cognitive impairment ( MCI ) due to Alzheimer′s disease.Methods All subjects were recruited from neurology ward and clinic of our hospital from December 2012 to September 2013.Using enzyme linked immunosorbent assay ( ELISA ) , the cerebrospinal fluid (CSF), serum and urinary levels of AD7C-NTP were tested for 88 elders, including 52 patients with MCI and 36 healthy controls.Statistical analysis was performed with SPSS 18.0.Results The CSF, serum and urinary levels of AD7C-NTP of two groups were (637 ±191) and (372 ±98); (499 ± 139) and (271 ±105) and ( 343 ±99 ) and ( 195 ±76 ) ng/L respectively.Using receiver operating characteristic ( ROC) curve, the optimal CSF cut-off point of AD7C-NTP for the diagnosis of MCI was 436.97 ng/L with a sensitivity of 86.7% and a specificity of 85.0%; the optimal serum cut-off point of AD7C-NTP for the diagnosis of MCI was 367.06 ng/L with a sensitivity of 76.7% and a specificity of 85.0%;the optimal cut-off point of AD7C-NTP in urine for the diagnosis of MCI was 268.57 ng/L with a sensitivity of 83.3% and a specificity of 90.0%.Conclusion Compared with the normal healthy control group, the MCI group has significantly elevated CSF , serum and urine levels of AD7C-NTP.The CSF and urinary levels of AD7C-NTP have high sensitivity and specificity with similar results.And AD7C-NTP may aid the diagnosis of MCI.