CAJ | 학술논문

目的：探讨性别对小鼠慢性低氧性肺动脉高压及肺动脉血管活性的影响。方法8周龄小鼠雌雄各30只，在雌雄两组中按随机数字表法随机分为常氧组与低氧组各15只，低氧组置于低氧舱（10％O2）中，4周后测定右心室收缩压与右心肥大指数，分离肺动脉环，观察高钾及U-46619诱发的肺动脉收缩强度，比较雌、雄小鼠低氧与常氧组间的差异。结果慢性低氧诱发了小鼠肺动脉高压，雌性和雄性常氧组右心室收缩压均显著低于同性别低氧组[（23.40±0.16）和（24.71±0.44）比（31.46±2.33）和（33.66±1.38） mmHg（1 mmHg＝0.133 kPa），P＝0.029和P＜0.001]；雌性和雄性常氧组右心肥大指数均显著低于同性别低氧组（0.260±0.015和0.272±0.007比0.354±0.012和0.367±0.012，P＝0.001和P＜0.001）；而雌性低氧组与雄性低氧组右心室收缩压、右心肥大指数差异均无统计学意义（ P＝0.447、0.471）。高钾、U-46619诱导的肺动脉血管收缩呈钙依赖性，撤除细胞外钙明显抑制血管环收缩。慢性低氧可显著增强高钾、U-46619诱发的肺动脉血管环收缩，收缩强度呈浓度依赖性，但在高钾、U-46619引起的收缩强度在雌、雄低氧组间差异均无统计学意义（P＝0.657、0.751）。结论性别与小鼠慢性低氧性肺动脉高压的发病、肺动脉高压的严重程度以及慢性低氧中的肺动脉血管活性均无显著相关性。
목적：탐토성별대소서만성저양성폐동맥고압급폐동맥혈관활성적영향。방법8주령소서자웅각30지，재자웅량조중안수궤수자표법수궤분위상양조여저양조각15지，저양조치우저양창（10％O2）중，4주후측정우심실수축압여우심비대지수，분리폐동맥배，관찰고갑급U-46619유발적폐동맥수축강도，비교자、웅소서저양여상양조간적차이。결과만성저양유발료소서폐동맥고압，자성화웅성상양조우심실수축압균현저저우동성별저양조[（23.40±0.16）화（24.71±0.44）비（31.46±2.33）화（33.66±1.38） mmHg（1 mmHg＝0.133 kPa），P＝0.029화P＜0.001]；자성화웅성상양조우심비대지수균현저저우동성별저양조（0.260±0.015화0.272±0.007비0.354±0.012화0.367±0.012，P＝0.001화P＜0.001）；이자성저양조여웅성저양조우심실수축압、우심비대지수차이균무통계학의의（ P＝0.447、0.471）。고갑、U-46619유도적폐동맥혈관수축정개의뢰성，철제세포외개명현억제혈관배수축。만성저양가현저증강고갑、U-46619유발적폐동맥혈관배수축，수축강도정농도의뢰성，단재고갑、U-46619인기적수축강도재자、웅저양조간차이균무통계학의의（P＝0.657、0.751）。결론성별여소서만성저양성폐동맥고압적발병、폐동맥고압적엄중정도이급만성저양중적폐동맥혈관활성균무현저상관성。
Objective To explore the effects of gender on pathology and pulmonary vascular reactivity in chronic hypoxic pulmonary hypertension ( HPH) in mice.Methods HPH was induced by a 4-week exposure of male and female mice ( 8 weeks old C57BL/6, n =15 each ) to chronic hypoxia (10%O2).Normoxic controls were established both in male and female groups (n =15 each).Right ventricular systolic pressure ( RVSP ) and Fulton index were detected.Isolated pulmonary artery ring contraction induced by high potassium ( K+) and U-46619 was recorded and compared between two groups.Results Chronic hypoxia induced pulmonary hypertension after 4 weeks.RVSP significantly increased both in hypoxic female and male groups ( normoxic vs hypoxic: ( 23.40 ±0.16 ) vs ( 31.46 ± 2.33) and (24.71 ±0.44) vs (33.66 ±1.38) mmHg (1 mmHg =0.133 kPa),P =0.029 and P<0.001).However,there were no inter-group differences (P=0.447).The Fulton index of hypoxic female and male mice was higher than normoxic counterparts (P=0.001,P<0.001).But there were no gender differences ( P =0.471 ).Contraction of high K +and U-46619 was calcium ion ( Ca2+)-dependent and decreased markedly without calcium (P<0.001,P=0.007).High K+and U-46619-induced contraction of pulmonary artery was concentration dependent and greater in chronic hypoxic female (P=0.002,P<0.001) and male mice ( both P<0.001).But no differences existed between male and female mice in high K +( P=0.657) or U-46619 ( P =0.751 )-induced contraction.Conclusion No significant correlation exists between gender and occurrence and severity of pulmonary hypertension or pulmonary artery vascular reactivity in HPH mice.