CAJ | 학술논문

目的：探讨miRNA-223、 miRNA-181 a在急性白血病（ AL）中的表达情况及其在AL临床预后中的意义。方法采用实时定量逆转录PCR （RT-PCR）方法检测42例AL患者miRNA-223、 miRNA-181a的表达，其中急性髓细胞白血病（ AML）患者35例，急性淋巴细胞白血病（ ALL）患者7例，以同期住院的5例非白血病患者中miRNA-223、 miRNA-181a的表达作为参照物，采用单因素方差分析（ANOVA）中的LSD法对多组样本均数间的比较进行分析。结果采集的样本中符合标准的共39例（其中AML 29例， ALL 6例，正常对照组4例）；29例AML中miRNA-223、 miRNA-181a相对表达水平的均数分别为6.65±1.29、9.7±2.20，6例ALL中miRNA-223、 miRNA-181a相对表达水平的均数分别为4.76±0.76、10.06±2.47； miRNA-223在AML、 ALL中的表达水平均低于正常对照组（ P＜0.01）， miRNA-223在AML中的表达水平明显高于ALL （ P＜0.01）， miRNA-181 a在AML、 ALL中的表达水平均高于正常对照组（ P＜0.01）， miRNA-181a在AML、 ALL中的表达差异无统计学意义； miRNA-181a在预后好分组中的表达水平明显高于预后中等组，差异有统计学意义（ P＜0.05），与APL组差异无统计学意义；此外， miRNA-223、 miRNA-181 a在初治、复发组及不同年龄及性别间无明显差异。结论 miRNA-223、 miRNA-181 a的表达与AL关系密切相关，可作为AL诊断的新标准，尤其在AML、ALL鉴别诊断中具有重要意义； miRNA-181 a可能在AML预后中具有重要价值，与患者临床疗效和长期生存有着直接关系； miRNA-223、 miRNA-181 a的表达与年龄、性别、初治与复发无关。
목적：탐토miRNA-223、 miRNA-181 a재급성백혈병（ AL）중적표체정황급기재AL림상예후중적의의。방법채용실시정량역전록PCR （RT-PCR）방법검측42례AL환자miRNA-223、 miRNA-181a적표체，기중급성수세포백혈병（ AML）환자35례，급성림파세포백혈병（ ALL）환자7례，이동기주원적5례비백혈병환자중miRNA-223、 miRNA-181a적표체작위삼조물，채용단인소방차분석（ANOVA）중적LSD법대다조양본균수간적비교진행분석。결과채집적양본중부합표준적공39례（기중AML 29례， ALL 6례，정상대조조4례）；29례AML중miRNA-223、 miRNA-181a상대표체수평적균수분별위6.65±1.29、9.7±2.20，6례ALL중miRNA-223、 miRNA-181a상대표체수평적균수분별위4.76±0.76、10.06±2.47； miRNA-223재AML、 ALL중적표체수평균저우정상대조조（ P＜0.01）， miRNA-223재AML중적표체수평명현고우ALL （ P＜0.01）， miRNA-181 a재AML、 ALL중적표체수평균고우정상대조조（ P＜0.01）， miRNA-181a재AML、 ALL중적표체차이무통계학의의； miRNA-181a재예후호분조중적표체수평명현고우예후중등조，차이유통계학의의（ P＜0.05），여APL조차이무통계학의의；차외， miRNA-223、 miRNA-181 a재초치、복발조급불동년령급성별간무명현차이。결론 miRNA-223、 miRNA-181 a적표체여AL관계밀절상관，가작위AL진단적신표준，우기재AML、ALL감별진단중구유중요의의； miRNA-181 a가능재AML예후중구유중요개치，여환자림상료효화장기생존유착직접관계； miRNA-223、 miRNA-181 a적표체여년령、성별、초치여복발무관。
Objective To investigate the expression of miRNA-223, miRNA-181a in acute leukemia (AL) and its prognostic value in AL.Methods The real-time quantitative analysis of variance ( ANOVA) was adopted to detect the expression of miRNA-223, miRNA-181a of 42 patients, including 35 cases with acute leukemia, 7 cases of acute lymphocytic leukemia, and 5 cases of non-leukemia patients at the same period in hospital as reference to analyze multiple sets of the sample mean by LSD of ANOVA.Results There were a total of 39 cases conforming to the standard in the collection of specimens ( including 29 AML patients , 6 ALL patients and 4 non-leukemia patients);the relative expression levels of miRNA-223, miRNA-181a in 29 AML patients were 6.65±1.29, 9.7 ±2.20 , and 4.76 ±0.76 , 10.06 ±2.47 in 6 ALL patients;the expression levels of miRNA-223 in AML and ALL were lower than those in normal control group ( P<0.01 ) , and the expression level of miRNA-223 in AML was significantly higher than that of ALL ( P<0.01 ) , the expression level of miRNA-181 a in AML and ALL were higher than those in normal control group ( P<0.01 );there was no significant difference of miRNA-181 a expression in AML and ALL;the expression level of miRNA-181 a in good prognosis group was higher than in middle prognosis group, the difference was statistically significant ( P<0.05 ) , not significantly different from APL group.In addition, the expression of miRNA-223 and miRNA-181a was not significantly different from the initial treatment group and the relapse group, the same in different age and gender.Conclusion The expression of miRNA-223, miRNA-181a was closely re-lated to AL, which can be used as a new standard in diagnosis of AL, especially significant in the differential diagnosis of AML and ALL, miRNA-181a may play an important role in the prognosis of AML, having a direct relationship with the long-term survival and the clinical efficacy;the expression of miRNA-223, miRNA-181a has nothing to do with age, gender, the initial treatment and the re-lapse.