CAJ | 학술논문

　　目的了解南宁市腹泻人群中空肠弯曲菌的感染流行状况。方法在南宁市对腹泻病例进行流行病学调查并采集粪便标本，进行实验室空弯菌的分离培养、PCR鉴定以及脉冲场凝胶电泳分型（PFGE）分析。结果在775份粪便样品中,3份空肠弯曲菌阳性，平均阳性率0.39%。所有生化结果阳性的菌株其PCR复核鉴定结果均为阳性。3株空肠弯曲菌经Cluster分析后得到3个PFGE带型。结论南宁市存在空肠弯曲菌感染病例，本研究结果为正确评价南宁市腹泻人群弯曲菌的流行状况和制定防控措施提供了科学依据。
　　목적료해남저시복사인군중공장만곡균적감염류행상황。방법재남저시대복사병례진행류행병학조사병채집분편표본，진행실험실공만균적분리배양、PCR감정이급맥충장응효전영분형（PFGE）분석。결과재775빈분편양품중,3빈공장만곡균양성，평균양성솔0.39%。소유생화결과양성적균주기PCR복핵감정결과균위양성。3주공장만곡균경Cluster분석후득도3개PFGE대형。결론남저시존재공장만곡균감염병례，본연구결과위정학평개남저시복사인군만곡균적류행상황화제정방공조시제공료과학의거。
Objective To determine the infectious status of campylobacter jejuni in the patients with diarrhea who lived in Nanning. Methods Epidemiologic investigation was conducted on the diarrheal patients living in Nanning, Their stool samples were collected and cultured for bacterial pathogens and were analyzed by means of Polymerase chain reaction (PCR) and Pulsed field gel electrophoresis (PFGE). Results Campylobacter jejuni was isolated from 3 of 775 stool samples with a positive rate of 0.39%. All positive isolates for biochemical tests were also positive for PCR reconfirmation. The PFGE patterns of 3 Campylobacter jejuni isolates belonged to 3 different clusters. Conclusion The cases infected with campylobacter jejuni have been identified in Nanning.The results of this study have provided scientific basis for appropriate evaluation of epidemic status of campylobacter jejuni in diarrheal patients in Nanning and for developing control measures.