CAJ | 학술논문

目的探讨地西他滨(DAC)对人胆管癌CCLP1细胞增殖的影响及其可能的作用机制.方法采用CCK-8法检测细胞生长抑制率;应用流式细胞仪检测细胞周期变化及凋亡发生情况;荧光显微镜观察经DAC作用后细胞的自噬现象.体内试验采用裸鼠CCLP1细胞皮下种植瘤模型,观察DAC对瘤体生长的影响.结果 DAC对人胆管癌CCLP1细胞的生长抑制作用呈剂量和时间依赖性(P＜0.05).流式细胞术检测显示,随着药物浓度的增加,CCLP1细胞的细胞周期呈现明显的G2/M阻滞,且凋亡细胞明显增多.荧光显微镜下观察到,经250 μmol/L DAC作用CCLP1细胞后,有明显的自噬现象发生.体内试验证明:当DAC腹腔注射剂量为0.8 mg/kg时,每周给药6次,总给药时间为2周,对裸鼠CCLP1细胞皮下移植瘤的生长有明显的抑制作用.结论体内、外实验证明,DAC对胆管癌的生长有明显的抑制作用.
목적 탐토지서타빈(DAC)대인담관암CCLP1세포증식적영향급기가능적작용궤제.방법 채용CCK-8법검측세포생장억제솔;응용류식세포의검측세포주기변화급조망발생정황;형광현미경관찰경DAC작용후세포적자서현상.체내시험채용라서CCLP1세포피하충식류모형,관찰DAC대류체생장적영향.결과 DAC대인담관암CCLP1세포적생장억제작용정제량화시간의뢰성(P＜0.05).류식세포술검측현시,수착약물농도적증가,CCLP1세포적세포주기정현명현적G2/M조체,차조망세포명현증다.형광현미경하관찰도,경250 μmol/L DAC작용CCLP1세포후,유명현적자서현상발생.체내시험증명:당DAC복강주사제량위0.8 mg/kg시,매주급약6차,총급약시간위2주,대라서CCLP1세포피하이식류적생장유명현적억제작용.결론 체내、외실험증명,DAC대담관암적생장유명현적억제작용.
Objective To investigate the effect and mechanism of decitabine (DAC) on the proliferation of human cholangiocarcinoma CCLP1 cells in vitro and in vivo.Methods After treated with various concentrations of DAC,cell growth inhibition rates were determined by CCK-8 assay.Cell cycle and cell apoptosis were analyzed by flow cytometry.Cell autophagy was observed under fluorescence microscope.The effect of DAC on the growth of cholangiocarcinoma in vivo was determined in a CCLP1 mice xenograft model.Results The proliferation rate of CCLP1 cells in the DAC-treated group decreased in a time-concentrated dependent manner.After treatment with DAC,the cell cycle of CCLP1 cells was arrested at the G2/M phase.The apoptosis rate was significantly higher in the treatment group over the control group.Cell autophagy was observed after treatment with DAC in CCLP1 cells.The tumor growth of implanted CCLP1 cells significantly slowed down after the mice were treated with 0.8 mg/kg DAC,6 times weekly for 2 weeks.Conclusion DAC can inhibit the proliferation of cholangiocarcinoma in vitro and in vivo.