CAJ | 학술논문

采用微升（μL）级微量指血为检测样品,在未对样品去蛋白的条件下,用邻苯二甲醛（OPA）荧光衍生化法测定了微量静脉血中还原型谷胱甘肽（GSH）的含量.利用标准曲线法和标准加入法进行3次测定的均值分别为4.415μmol/L和5.417 5μmol/L,加标回收率为95.28%～97.18%,检测限为3.6×10-8 mol/L,可基本满足微量成分分析所要求的准确度.本研究为灵敏、简便和快速测定微量血样中GSH的含量提供了实验和方法学的依据.
채용미승（μL）급미량지혈위검측양품,재미대양품거단백적조건하,용린분이갑철（OPA）형광연생화법측정료미량정맥혈중환원형곡광감태（GSH）적함량.이용표준곡선법화표준가입법진행3차측정적균치분별위4.415μmol/L화5.417 5μmol/L,가표회수솔위95.28%～97.18%,검측한위3.6×10-8 mol/L,가기본만족미량성분분석소요구적준학도.본연구위령민、간편화쾌속측정미량혈양중GSH적함량제공료실험화방법학적의거.
Reduced glutathione is sensitively determinined by fluorescent spectrometry using o-phthalaldehyde （OPA） as fluorescent derivatizing agent and different calibration methods by micro sampling of finger blood without protein elimination. The average amounts of GSH determined by calibration and standard addition methods are 4. 415 μmol/L and 5. 417 5μmol/L respectively, recoveries are in the range of 95.28% 97.18%, detection limits is 3.6 × 10-8 mol/L. The methods is suitable for simple, rapid and sensitive determination of GSH in micro blood sample.