CAJ | 학술논문

为了建立红掌新品种‘Fiesta’和‘Altimo’离体繁殖体系，本文以叶柄为试验材料，进行外植体消毒、愈伤诱导和植株再生试验。结果表明：最佳消毒方法为50 mg/L制霉菌素浸泡20 min、70%乙醇浸泡30 s、0.1%升汞浸泡5 min、无菌水清洗6次，该方法处理的外植体污染率和褐化率均较低；综合考虑出愈率和愈伤活力，‘Fiesta’和‘Altimo’的最佳愈伤诱导培养基分别为1/2MS+6-BA 1.00 mg/L+2,4-D 0.2 mg/L和1/2MS+6-BA 2.00 mg/L+2,4-D 0.10 mg/L；最适分化培养基均为1/2MS (蔗糖25.00 g/L)+6-BA 0.50 mg/L+2ip 0.10 mg/L+NAA 0.05 mg/L。本研究建立了红掌品种‘Fiesta’和‘Altimo’的愈伤诱导和再生体系，结果为种苗快繁和遗传转化研究提供了技术参考。
위료건립홍장신품충‘Fiesta’화‘Altimo’리체번식체계，본문이협병위시험재료，진행외식체소독、유상유도화식주재생시험。결과표명：최가소독방법위50 mg/L제매균소침포20 min、70%을순침포30 s、0.1%승홍침포5 min、무균수청세6차，해방법처리적외식체오염솔화갈화솔균교저；종합고필출유솔화유상활력，‘Fiesta’화‘Altimo’적최가유상유도배양기분별위1/2MS+6-BA 1.00 mg/L+2,4-D 0.2 mg/L화1/2MS+6-BA 2.00 mg/L+2,4-D 0.10 mg/L；최괄분화배양기균위1/2MS (자당25.00 g/L)+6-BA 0.50 mg/L+2ip 0.10 mg/L+NAA 0.05 mg/L。본연구건립료홍장품충‘Fiesta’화‘Altimo’적유상유도화재생체계，결과위충묘쾌번화유전전화연구제공료기술삼고。
To establish the in vitro propagation system of Anthurium andraeanum cv.‘Fiesta’and‘Altimo’, in this study, we employed petiole as explant, to optimize protocol of disinfection, callus induction, and plantlet regeneration in this study. The results showed that the best disinfection method was sequentially immersing for 20 min in 50 mg/L Nystatin, 30 s in 70%Ethanol, 5 min in 0.1%HgCl2, and then washing 6 times in sterile water.‘Fiesta’and‘Altimo’displayed the highest frequencies of callus induction on improved medium:1/2MS+6-BA 1.00 mg/L+2,4-D 0.2 mg/L and 1/2MS+6-BA 2.00 mg/L+2,4-D 0.10 mg/L respectively. For both cultivars, the optimum shoots induction medium were 1/2MS (25.00 g/L sucrose)+6-BA 0.50 mg/L+2ip 0.10 mg/L+NAA 0.50 mg/L. This study has successfully outlined a rapid, high frequency in vitro propagation for Anthurium, it provide technical reference for Seedling Production and genetic transformation.