CAJ | 학술논문

DNA分子在生物体内受到物理、化学等因素的影响，如X-射线和药物，会发生不同程度与类型的碱基损伤，诱发基因突变或细胞损害，引发包括癌症在内的各种疾病。因此研究DNA损伤与修复机制至关重要。但在DNA损伤修复机制研究过程中，目前最大的困难在于如何有效架构碱基损伤的DNA与用于修复DNA损伤修复酶的瞬态复合物，以探讨这些酶如何特异性识别损伤位点。本文综述利用化学交联法通过二硫键的方式实现酶与碱基损伤的DNA瞬态复合物的形成的相关研究进展，以期为其它DNA损伤或DNA碱基化学修饰机制研究提供参考。
DNA분자재생물체내수도물리、화학등인소적영향，여X-사선화약물，회발생불동정도여류형적감기손상，유발기인돌변혹세포손해，인발포괄암증재내적각충질병。인차연구DNA손상여수복궤제지관중요。단재DNA손상수복궤제연구과정중，목전최대적곤난재우여하유효가구감기손상적DNA여용우수복DNA손상수복매적순태복합물，이탐토저사매여하특이성식별손상위점。본문종술이용화학교련법통과이류건적방식실현매여감기손상적DNA순태복합물적형성적상관연구진전，이기위기타DNA손상혹DNA감기화학수식궤제연구제공삼고。
Cellular DNA is constantly subjected to modifications by intracellular and extracellular chemicals, which can result in all kinds of damage, and then cause various diseases including cancer. To understand pro-tein/DNA interactions and the damage repair mechanism, a structure of the protein/DNA complex is desirable. However, transient protein/DNA complexes are almost impossible to obtain for structural studies using traditional methods for several reasons. In this paper, a detail review on the research progress that previous studies have used disulfide crosslinking to trap distinct structural states of a protein/DNA interaction, especially on known direct DNA repair proteins, which are engaged in alkylation DNA damage repair. This method, an equilibrium process that relies on protein/DNA interactions, enables us to trap and isolate homogeneous protein/DNA complexes for structural characterization.