CAJ | 학술논문

采用Real-time PCR和原位杂交检测TEAD1在围着床期小鼠子宫及人工诱导蜕膜化组织中的表达情况。Real-time PCR结果显示，在妊娠1~8 d的小鼠子宫中，TEAD1 mRNA的表达随妊娠天数的增加逐渐增强；原位杂交结果显示，TEAD1 mRNA强表达于5~8 d小鼠子宫蜕膜细胞及胚胎上，TEAD1 mRNA强表达于人工诱导蜕膜化组织中。推测TEAD1参与调节子宫基质蜕膜化过程，在胚胎着床过程发挥重要作用。
채용Real-time PCR화원위잡교검측TEAD1재위착상기소서자궁급인공유도세막화조직중적표체정황。Real-time PCR결과현시，재임신1~8 d적소서자궁중，TEAD1 mRNA적표체수임신천수적증가축점증강；원위잡교결과현시，TEAD1 mRNA강표체우5~8 d소서자궁세막세포급배태상，TEAD1 mRNA강표체우인공유도세막화조직중。추측TEAD1삼여조절자궁기질세막화과정，재배태착상과정발휘중요작용。
Real-time PCR and in situ hybridization were used to detect the expresstion of TEAD1 in peri-implantation uterus of mice and in artificial decidual cells. Real-time PCR results showed that the expression level of TEAD1 mRNA was gradual y increased in pregnant mice 1-8 d uterus; in situ hybridization showed that, TEAD1 strongly expressed in uterine decidual cells and embryos from pregnant 5-8 d and artificial decidual cells. Inferred TEAD1 might be involved in regulating uterine stromal decidualization and play an important role in embryo implantation process.