CAJ | 학술논문

为建立猪传染性胃肠炎病毒（TGEV）和猪流行性腹泻病毒（PEDV）的快速检测方法，参照GenBank已发表序列，根据TGEV和PEDV的S蛋白基因结构各设计1套特异性通用引物，扩增目的带分别为426和583 bp。扩增产物经琼脂糖凝胶电泳，紫外光下于500 bp上、下各有一条电泳带，达到区分和鉴别目的。经优化确立最佳反应体系：引物、MgCl2和dNTP浓度分别为8、9和14μmol·L-1，退火温度54℃。该方法在捕获信息和复杂基因分析方面具有快速、敏感、特异、低成本等优点，可为TGEV和PEDV检测及TGE和PED流行病学调查等提供技术支持。
위건립저전염성위장염병독（TGEV）화저류행성복사병독（PEDV）적쾌속검측방법，삼조GenBank이발표서렬，근거TGEV화PEDV적S단백기인결구각설계1투특이성통용인물，확증목적대분별위426화583 bp。확증산물경경지당응효전영，자외광하우500 bp상、하각유일조전영대，체도구분화감별목적。경우화학립최가반응체계：인물、MgCl2화dNTP농도분별위8、9화14μmol·L-1，퇴화온도54℃。해방법재포획신식화복잡기인분석방면구유쾌속、민감、특이、저성본등우점，가위TGEV화PEDV검측급TGE화PED류행병학조사등제공기술지지。
Arapidduplex RT-PCR assay was developed for simultaneous detection and discrimination of transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) using the primers designed basing on their S protein gene reported in GenBank. The producedemonstrated after agarose gels electrophoresis stained with ethidium bromide that one specific cDNA band of 426 bp for TGEV and another specific cDNA band of 583 bp for PEDV up and down the 500bp indicating band. Optimization reaction system:concentration of primers, MgCl2 and dNTP were 8, 9 and 14μmol·L-1, respectively, and renaturation temperature was 54 ℃. This method took the advantages of rapid, sensitive, specific, andlow cost, in capturing information, analyzing the complexity of gene, which could provide technical support for detection of TGEV and PEDV, and epidemiological investigation of TGE and PED, etc.