CAJ | 학술논문

matK基因是陆地植物核心条码候选片段之一，文章通过克隆获得22个核桃品种matK基因近3'端长度为726 bp EST序列，运用序列分析技术探索matK在核桃种间关系研究中的价值。结果表明，在所测的matK基因的核苷酸序列中发生变异的核桃品种均属早实核桃类群，而晚实核桃类群无变异发生；推导氨基酸序列分析显示薄壳香由于发生突变，提前终止翻译而只编码173个氨基酸，而其他品种均编码234个氨基酸；采用近邻接树法和最大简约法构建的系统进化树显示，22个品种核桃分为5个组，其中扎343与其他21个品种有明显差异，8个新选育的品种中金薄丰1号较为突出；而辽核1号与西扶1号亲缘关系最近。对matK基因深入研究将为核桃种质资源保护、杂交育种及开发运用提供分子生物学依据。
matK기인시륙지식물핵심조마후선편단지일，문장통과극륭획득22개핵도품충matK기인근3'단장도위726 bp EST서렬，운용서렬분석기술탐색matK재핵도충간관계연구중적개치。결과표명，재소측적matK기인적핵감산서렬중발생변이적핵도품충균속조실핵도류군，이만실핵도류군무변이발생；추도안기산서렬분석현시박각향유우발생돌변，제전종지번역이지편마173개안기산，이기타품충균편마234개안기산；채용근린접수법화최대간약법구건적계통진화수현시，22개품충핵도분위5개조，기중찰343여기타21개품충유명현차이，8개신선육적품충중금박봉1호교위돌출；이료핵1호여서부1호친연관계최근。대matK기인심입연구장위핵도충질자원보호、잡교육충급개발운용제공분자생물학의거。
matK gene is known as one of the core bar code segments for the candidated land plants. In this paper, we acquired the matK genes 726 bp EST which located in the 3' end from 22 walnut varieties and explored the value of the matK gene on establishing walnut interspecies relation by the sequence analysis. The results demonstrate that the nucleotide sequence of early bearing walnuts matK genes' mutation has been detected, but the later mature walnuts were opposite. Amino Acid Sequence Analysis indicated that nuthin shel had promotes premature translational-termination and generates 173 amino acids because of mutations, which was extensively different the normal products 234 amino acids. Neighbours pick Neighbor-Joining and Maxium Parsimony was used to construct the system of evolutionary tree and 22 walnut varieties were divided into five groups, we concluded that Zha343 had distinct differences with other 21 walnut varieties, Jinbofeng1 gained an advantage over the other varieties among the novel varieties and Liaohe1 and xifu1 were the closest cousin. In a word, the further research in matK genes provide the a molecular basis for walnut germplasm resourced protection, development and application of hybridization breeding.