CAJ | 학술논문

目的：从致病菌角度分析复发性外阴阴道念珠菌病( RVVC)复发的危险因素。方法收集30例RVVC患者阴道和肛管的菌株,经白念珠菌特异性引物PCR鉴定有17例为白念珠菌并入选研究。三色荧光分别标记保守基因CDC3、EF3和HIS3微卫星序列引物,PCR扩增,产物行聚丙烯酰胺凝胶电泳。 Gene Scan软件扫描获取片段精确长度,Genotype软件基因分型。结果分离自17例患者阴道和肛管的白念珠菌,采用微卫星基因分型法,基因型彼此相同。结论 RVVC患者阴道的菌株可能从肛管移行而来,这可能是该病反复发作的重要因素之一。
목적：종치병균각도분석복발성외음음도념주균병( RVVC)복발적위험인소。방법수집30례RVVC환자음도화항관적균주,경백념주균특이성인물PCR감정유17례위백념주균병입선연구。삼색형광분별표기보수기인CDC3、EF3화HIS3미위성서렬인물,PCR확증,산물행취병희선알응효전영。 Gene Scan연건소묘획취편단정학장도,Genotype연건기인분형。결과분리자17례환자음도화항관적백념주균,채용미위성기인분형법,기인형피차상동。결론 RVVC환자음도적균주가능종항관이행이래,저가능시해병반복발작적중요인소지일。
Objective To analyze the risk factors of pathogens in relapse of recurrent vulvovaginal candidiasis ( RVVC) . Methods Candida strains were isolated from the vaginas and anal canals of 30 patients with RVVC. Can-dida strains from 17 patients were identified as candida albicans by specific primers. The house keeping genes CDC3, EF3 and HIS3 was featured with microsatellite sequence. The 3 sites of house keeping gene were amplified by specific primers, which were labeled with different fluorescein. PCR products were run on polyacrylamide gel and sizes of products were scanned by GeneScan software and identified by Genotype software. Results The genotypes of 17 pairs of candida albicans strains were same. Conclusion In patients with RVVC, the strains could migrate form anal canal to vagina, which might be the important causes for the recurrence of RVVC.