CAJ | 학술논문

【目的】对松材线虫类甜蛋白（TLP-1）结构进行分析，进一步为其功能研究奠定基础。【方法】通过对松材线虫（Bursaphelenchus xylophilus ）类甜蛋白（TLP-1）设计特异性引物，运用RT-PCR技术克隆该基因全长cDNA序列。运用相关生物学软件进行分析及三维结构构建。【结果】该基因全长为441 bp，编码146个氨基酸。NCBI比对发现该基因属于TLP超基因家族。PROCHECK分析显示蛋白结构建合理。【结论】该蛋白不论从序列分析以及蛋白构建结果显示，相对于动物源类甜蛋白，其与植物更为相似。
【목적】대송재선충류첨단백（TLP-1）결구진행분석，진일보위기공능연구전정기출。【방법】통과대송재선충（Bursaphelenchus xylophilus ）류첨단백（TLP-1）설계특이성인물，운용RT-PCR기술극륭해기인전장cDNA서렬。운용상관생물학연건진행분석급삼유결구구건。【결과】해기인전장위441 bp，편마146개안기산。NCBI비대발현해기인속우TLP초기인가족。PROCHECK분석현시단백결구건합리。【결론】해단백불론종서렬분석이급단백구건결과현시，상대우동물원류첨단백，기여식물경위상사。
Obj ective]The aim of the study was to analyze the structure of thaumatin protein (TLP-1 )in pine wood nematode,and to build the basis for knowing its function.[Method]In this study,we designed the specific primers for TLP-1 of B.xylophilus,Full-length cDNA se-quence of the gene was cloned using RT-PCR.Three-dimensional structure was analyzed and con-structed by relevant biological softwares.[Results]The full length of cDNA sequence of the gene was 441 bp,encoding 146 amino acids.The protein belonged to TLP supergene family according to NCB.PROCHECK analysis showed that the protein structure was reasonable.[Conclusion]Either in sequence analysis or protein structure,this protein was similar to plants relative to animals.