CAJ | 학술논문

【目的】探索不同因素对青脆李花粉生活力及萌发率的影响。【方法】以青脆李花粉为试材，通过TTC法研究了不同发育阶段、干燥方式、贮藏条件、贮藏时间4个因素对青脆李花粉生活力的影响，并采用培养基萌发法观察花粉萌发过程及植物生长调节剂对花粉萌发的作用。【结果】青脆李盛花期花粉生活力，明显高于初花期和末花期；室内自然阴干的花粉生活力平均高于干燥器干燥的花粉生活力；常温、0、4及-18℃条件下贮藏的花粉平均生活力随温度降低呈升高趋势；花粉生活力随贮藏时间的增长逐渐减弱，-18℃贮藏30 d的花粉生活力可达35．3％；6-BA对花粉萌发有抑制作用，适宜浓度的2，4-D（20 mg/L）可促进花粉萌发。【结论】盛花期花粉自然阴干是花粉的最佳采集方式，而长时间贮藏则以低温保存为宜。
【목적】탐색불동인소대청취리화분생활력급맹발솔적영향。【방법】이청취리화분위시재，통과TTC법연구료불동발육계단、간조방식、저장조건、저장시간4개인소대청취리화분생활력적영향，병채용배양기맹발법관찰화분맹발과정급식물생장조절제대화분맹발적작용。【결과】청취리성화기화분생활력，명현고우초화기화말화기；실내자연음간적화분생활력평균고우간조기간조적화분생활력；상온、0、4급-18℃조건하저장적화분평균생활력수온도강저정승고추세；화분생활력수저장시간적증장축점감약，-18℃저장30 d적화분생활력가체35．3％；6-BA대화분맹발유억제작용，괄의농도적2，4-D（20 mg/L）가촉진화분맹발。【결론】성화기화분자연음간시화분적최가채집방식，이장시간저장칙이저온보존위의。
Obj ective]The aim of the study was to investigate effects of different factors on pollen viability and germination rate of Qingcui plum.[Method]The effects of development stage,des-iccating methods,storage condition and time on pollen viability of Qingcui plum were investigated by TTC method.The pollen germination process and effect of growth substance on pollen activity were observed based on medium.[Results]Pollen viability of Qingcui plum in the flowering sea-son was higher compared to the beginning and ending of blooming period.Pollen viability by natu-ral drying was higher than that by drying in desiccator.Pollen viability tended to increase with de-creasing temperature (room temperature,0,4 and -18 ℃).In addition,pollen viability tended to decrease with storage time.The pollen viability in -18 ℃for 30 days was 35. 3%.Pollen ger-mination was inhibited by 6-BA but 20 mg/L of 2 ,4-D stimulated pollen germination.[Conclu-sion]Natural drying during the flowering season was the best method for pollen collection.How-ever,low temperature was optimal for long-term storage.