四川农业大学学报
四川農業大學學報
사천농업대학학보
JOURNAL OF SICHUAN AGRICULTURAL UNIVERSITY
2014年
3期
283-287
,共5页
大花蕙兰%耐寒性%主成分分析%综合评价
大花蕙蘭%耐寒性%主成分分析%綜閤評價
대화혜란%내한성%주성분분석%종합평개
Cymbidium hybridum%cold resistance%principal component analysis%comprehensive evaluation
【目的】比较‘黄金岁月’(Cymbidium Lovely Moon'Crescent')、‘红霞’(Cymbidium Royal Red'Princess Nobu-ko')和‘晨辉’(Cymbidium Twilight Moon'Daylight')3个品种大花蕙兰耐寒性的强弱,筛选大花蕙兰耐寒性鉴定的生理指标。【方法】采用人工模拟低温的方法,设置10、5、0和-5℃共4个低温处理,探讨其对3个品种大花蕙兰地上部和地下部丙二醛(MDA)、膜透性(CMP)、可溶性蛋白质(SP)、可溶性糖(SS)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等7项生理指标的影响,利用主成分分析、隶属函数法和逐步回归分析,对这7个耐寒性生理指标进行综合评价。【结果】3个不同品种大花蕙兰在受到低温胁迫时,3种酶活性有不同程度升高,其中SOD和POD活性增加幅度最为明显,其耐寒性强弱的排序为:‘黄金岁月’>‘红霞’>‘晨辉’。【结论】从不同生理指标的变化趋势来看,细胞膜透性、SOD和POD活性的变化趋势可反映出不同品种的大花蕙兰组培苗的耐寒性的强弱,因此,可将细胞膜通透性、SOD和POD活性作为其耐寒性的鉴定指标。地上部和地下部在反映组培苗耐寒性能方面存在差异性。
【目的】比較‘黃金歲月’(Cymbidium Lovely Moon'Crescent')、‘紅霞’(Cymbidium Royal Red'Princess Nobu-ko')和‘晨輝’(Cymbidium Twilight Moon'Daylight')3箇品種大花蕙蘭耐寒性的彊弱,篩選大花蕙蘭耐寒性鑒定的生理指標。【方法】採用人工模擬低溫的方法,設置10、5、0和-5℃共4箇低溫處理,探討其對3箇品種大花蕙蘭地上部和地下部丙二醛(MDA)、膜透性(CMP)、可溶性蛋白質(SP)、可溶性糖(SS)、超氧化物歧化酶(SOD)、過氧化物酶(POD)和過氧化氫酶(CAT)等7項生理指標的影響,利用主成分分析、隸屬函數法和逐步迴歸分析,對這7箇耐寒性生理指標進行綜閤評價。【結果】3箇不同品種大花蕙蘭在受到低溫脅迫時,3種酶活性有不同程度升高,其中SOD和POD活性增加幅度最為明顯,其耐寒性彊弱的排序為:‘黃金歲月’>‘紅霞’>‘晨輝’。【結論】從不同生理指標的變化趨勢來看,細胞膜透性、SOD和POD活性的變化趨勢可反映齣不同品種的大花蕙蘭組培苗的耐寒性的彊弱,因此,可將細胞膜通透性、SOD和POD活性作為其耐寒性的鑒定指標。地上部和地下部在反映組培苗耐寒性能方麵存在差異性。
【목적】비교‘황금세월’(Cymbidium Lovely Moon'Crescent')、‘홍하’(Cymbidium Royal Red'Princess Nobu-ko')화‘신휘’(Cymbidium Twilight Moon'Daylight')3개품충대화혜란내한성적강약,사선대화혜란내한성감정적생리지표。【방법】채용인공모의저온적방법,설치10、5、0화-5℃공4개저온처리,탐토기대3개품충대화혜란지상부화지하부병이철(MDA)、막투성(CMP)、가용성단백질(SP)、가용성당(SS)、초양화물기화매(SOD)、과양화물매(POD)화과양화경매(CAT)등7항생리지표적영향,이용주성분분석、대속함수법화축보회귀분석,대저7개내한성생리지표진행종합평개。【결과】3개불동품충대화혜란재수도저온협박시,3충매활성유불동정도승고,기중SOD화POD활성증가폭도최위명현,기내한성강약적배서위:‘황금세월’>‘홍하’>‘신휘’。【결론】종불동생리지표적변화추세래간,세포막투성、SOD화POD활성적변화추세가반영출불동품충적대화혜란조배묘적내한성적강약,인차,가장세포막통투성、SOD화POD활성작위기내한성적감정지표。지상부화지하부재반영조배묘내한성능방면존재차이성。
[Obj ective]The aim of the study was to compare cold resistance among three varieties of C.hybridum and to select physiological indicators of cold-resistance of C.Hybridum.[Meth-od]This study included four low temperature treatments (10,5,0 and -5 ℃)by artificial simu-lation method.The effects of low temperatures on malondialdehyde (MDA),Membrane permea-bility (CMP),soluble protein (SP),soluble sugar (SS),superoxide dismutase (SOD),peroxi-dase (POD)and catalase (CAT)activities of aboveground and underground parts of three C.hy-bridum varieties were investigated.The seven cold-resistance physiological indexes were compre-hensively evaluated using principal component analysis,membership function analysis and step-wise regression analysis.[Results]Three C.hybridum when subjected to cold stress,there are three kinds of activity increased to varying degrees,of which the magnitude of SOD and POD ac-tivity increased the most obvious,the order of cold-resistance capacity of varieties was Lovely C. Moon'Crescent'> C.Royal Red'Princess Nobuko'> C.Twilight Moon'Daylight'.[Conclusion]CMP,SOD and POD activities were significantly associated with cold resistance,and thought be the indexes to identify cold resistance in C.hybridum.There were differences in cold-resistance between aboveground and underground components.