CAJ | 학술논문

【目的】比较稻瘟病抗性基因Pi-km的不同分子标记，筛选能真实反映其表型的分子标记用于辅助育种选择。【方法】本研究利用与稻瘟病抗性基因Pi-km紧密连锁的1个SNP标记及Pi-km基因内部的2个Indel 标记，对62份2012年长江上游国家水稻区域试验材料和97份陕西省水稻区试材料进行了基因型分析，并结合这些材料的稻瘟病抗性的田间鉴定结果，分析了基因型与抗病性之间的关系，对不同的分子标记辅助育种选择进行了评价。【结果】2类标记检测的基因型不完全一致，基于Pi-km基因序列设计的标记比与其连锁的标记作为辅助育种选择更准确。【结论】Pi-km基因的稻瘟病抗性与基因型有关，基因型为 M1-M2-的材料比其他基因型材料抗病性趋势更强。
【목적】비교도온병항성기인Pi-km적불동분자표기，사선능진실반영기표형적분자표기용우보조육충선택。【방법】본연구이용여도온병항성기인Pi-km긴밀련쇄적1개SNP표기급Pi-km기인내부적2개Indel 표기，대62빈2012년장강상유국가수도구역시험재료화97빈합서성수도구시재료진행료기인형분석，병결합저사재료적도온병항성적전간감정결과，분석료기인형여항병성지간적관계，대불동적분자표기보조육충선택진행료평개。【결과】2류표기검측적기인형불완전일치，기우Pi-km기인서렬설계적표기비여기련쇄적표기작위보조육충선택경준학。【결론】Pi-km기인적도온병항성여기인형유관，기인형위 M1-M2-적재료비기타기인형재료항병성추세경강。
Obj ective]The different molecular markers of resistant gene Pi-km of rice blast were compared.Selecting the markers that can represent true phenotype was helpful for breeding.[Method]The gene Pi-km was a main resistant allele of Pik locus to rice blast.The genotypes of Pi-km in 62 rice trials in 2012 in the upper Yangtze river and 97 rice trials in Shan'xi were analyzed using one SNP marker that was linked to Pi-km and two Indel markers within Pi-km.In combina-tion with field data of resistance of those lines to rice blast,the relationship between genotypes of those markers in Pi-km and disease resistance was evaluated.[Results]The results showed that genotyping results were not completely consistent between SNP and Indel markers.Indel markers within Pi-km were more accurate than the SNP marker that tightly linked to Pi-km in marker-as-sisted selection.[Conclusion]Rice blast resistance was associated with the genotypes identified by the markers.The resistance of lines with M1-M2-genotype was stronger than that of other gen-otypes.