热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
1期
48-53
,共6页
代龙军%黎瑜%康桂娟%聂智毅%段翠芳%曾日中
代龍軍%黎瑜%康桂娟%聶智毅%段翠芳%曾日中
대룡군%려유%강계연%섭지의%단취방%증일중
巴西橡胶树%橡胶粒子%蛋白质%RicinB_Lectin_2结构域
巴西橡膠樹%橡膠粒子%蛋白質%RicinB_Lectin_2結構域
파서상효수%상효입자%단백질%RicinB_Lectin_2결구역
Hevea brasiliensis%rubber particles%protein%RicinB_Lectin_2 domain
采用不同提取液,从洗涤过的巴西橡胶树橡胶粒子中提取蛋白质,并对其进行电泳分离与质谱鉴定。分离鉴定了2个含RicinB_Lectin_2结构域的糖基识别蛋白质,分别命名为HbRBLLP-1和HbRBLLP-2。这2个蛋白质可能是橡胶粒子膜蛋白质或是与橡胶粒子有较强的相互作用的蛋白质。对HbRBLLP-1及HbRBLLP-2进行生物信息学分析,结果表明它们的RicinB_Lectin_2结构域均仅有1个Q-x-W模体,而非蓖麻毒素B链所具有的(Q-x-W)3模体。
採用不同提取液,從洗滌過的巴西橡膠樹橡膠粒子中提取蛋白質,併對其進行電泳分離與質譜鑒定。分離鑒定瞭2箇含RicinB_Lectin_2結構域的糖基識彆蛋白質,分彆命名為HbRBLLP-1和HbRBLLP-2。這2箇蛋白質可能是橡膠粒子膜蛋白質或是與橡膠粒子有較彊的相互作用的蛋白質。對HbRBLLP-1及HbRBLLP-2進行生物信息學分析,結果錶明它們的RicinB_Lectin_2結構域均僅有1箇Q-x-W模體,而非蓖痳毒素B鏈所具有的(Q-x-W)3模體。
채용불동제취액,종세조과적파서상효수상효입자중제취단백질,병대기진행전영분리여질보감정。분리감정료2개함RicinB_Lectin_2결구역적당기식별단백질,분별명명위HbRBLLP-1화HbRBLLP-2。저2개단백질가능시상효입자막단백질혹시여상효입자유교강적상호작용적단백질。대HbRBLLP-1급HbRBLLP-2진행생물신식학분석,결과표명타문적RicinB_Lectin_2결구역균부유1개Q-x-W모체,이비비마독소B련소구유적(Q-x-W)3모체。
Proteins extracted with different buffers from washed Hevea brasiliensis rubber particles were subjected to electrophoresis and mass spectrometry analysis. Two carbohydrate binding RicinB_Lectin_2 domain containing proteins were isolated and identified, and these two proteins were designated as HbRBLLP-1 and HbRBLLP-2, respectively. These two proteins may be membrane proteins of rubber particles or proteins have a strong interaction with rubber particles. Bioinformatics analysis was also carried out on these two proteins. Bioinformatics analysis showed that HbRBLLP-1 and HbRBLLP-2 have only one QxW motif in their RicinB_Lectin_2 domain, respectively; whereas, ricin B chain has a (QxW)3 motif.