CAJ | 학술논문

采用微透析法获取3种杨树嫩茎质外体汁液的灌流液,流速1μL/min,探针截留分子量为30 kDa,无须处理直接通过原子吸收光谱(石墨炉法)测定样品中Na+, K+, Ca2+浓度,通过建立实时、无损检测离子浓度变化的实验技术,进行杨树生理生化的研究。此方法加标回收率在95.8%~103.1%之间。研究表明,吴屯杨、小胡杨和小美旱的Na+含量分别为1034~1156μg/L,1493~1611μg/L和1586~1703μg/L；K+含量分别为1012~1237μg/L,941~964μg/L和1095~1201μg/L；Ca2+含量分别为4976~5237μg/L,4786~5042μg/L和5893~6142μg/L,本方法为在植物应激反应中通过离子浓度变化来适应外界环境的研究提供可靠数据。
채용미투석법획취3충양수눈경질외체즙액적관류액,류속1μL/min,탐침절류분자량위30 kDa,무수처리직접통과원자흡수광보(석묵로법)측정양품중Na+, K+, Ca2+농도,통과건립실시、무손검측리자농도변화적실험기술,진행양수생리생화적연구。차방법가표회수솔재95.8%~103.1%지간。연구표명,오둔양、소호양화소미한적Na+함량분별위1034~1156μg/L,1493~1611μg/L화1586~1703μg/L；K+함량분별위1012~1237μg/L,941~964μg/L화1095~1201μg/L；Ca2+함량분별위4976~5237μg/L,4786~5042μg/L화5893~6142μg/L,본방법위재식물응격반응중통과리자농도변화래괄응외계배경적연구제공가고수거。
The dialysis for three species of poplar stems apoplast was obtained by microdialysis method, and then the concentration of Na+, K+, Ca2+ in the dialysis sample was directly measured by graphite furnace atomic absorption spectrometry ( GF-AAS) . The flow rate of perfusate was 1μL/min and the cut-off molecular weight of probe was 30 kDa. The real-time and non-destructive test technique established for the detection of ion concentration could be applied in research of poplar biochemical physiology. The recovery of the proposed method was 95. 8%-103. 1%. The contents of sodium ion in Populus wutunensis, Populus simonii and Salix matsudana were 1034-1156 μg/L, 1493-1611 μg/L and 1586-1703 μg/L; potassium ion were 1012-1237 μg/L, 941-964 μg/L and 1095-1201 μg/L; calcium ion were 4976-5237 μg/L, 4786-5042 μg/L and 5893-6142 μg/L. The method could provide reliable data for the study of ion concentration changes of plant response to adapt to all kinds of external environment.