CAJ | 학술논문

建立了以QuEChERS样品预处理方法、四级杆飞行时间串联质谱( Q-TOF MS)为检测手段,针对鱼肉中的四环素类、磺胺类、喹诺酮类、三苯甲烷类、雄激素、孕激素和糖皮质激素7类59种残留药物的快速筛查技术。通过正交实验得到优化条件为：Na2 EDTA-Mcllvaine为缓冲溶液,乙腈为提取剂,净化剂无水MgSO4、十八烷基键合硅胶(C18)和N-丙基乙二胺(PSA)的用量分别为每毫升提取液加入25,12.5和6.25 mg。对Q-TOF质谱筛查出的阳性样品,再用LC-MS/MS对样品进行确证。本方法对59种药物的检出下限为0.5~5.3μg/kg。本方法快速、简单、可靠,可筛查的渔药种类覆盖面广,灵敏度满足水产品药物残留检测的要求,适用于鱼类水产品中残留药物的快速测定。
건립료이QuEChERS양품예처리방법、사급간비행시간천련질보( Q-TOF MS)위검측수단,침대어육중적사배소류、광알류、규낙동류、삼분갑완류、웅격소、잉격소화당피질격소7류59충잔류약물적쾌속사사기술。통과정교실험득도우화조건위：Na2 EDTA-Mcllvaine위완충용액,을정위제취제,정화제무수MgSO4、십팔완기건합규효(C18)화N-병기을이알(PSA)적용량분별위매호승제취액가입25,12.5화6.25 mg。대Q-TOF질보사사출적양성양품,재용LC-MS/MS대양품진행학증。본방법대59충약물적검출하한위0.5~5.3μg/kg。본방법쾌속、간단、가고,가사사적어약충류복개면엄,령민도만족수산품약물잔류검측적요구,괄용우어류수산품중잔류약물적쾌속측정。
A high-throughput method for screening drug multi residues was developed by quadrupole-time-of-flight( Q-TOF) mass spectrometry together with QuEChERS sample preparation technique. It has been used for the determination of 57 drugs, such as tetracyclines ( TCs ) , sulfonamides ( SAs ) , quinolones ( QNs ) , triphenylmethanes (TPMs), estrogens (ETs), androgens (AGs) and glucocorticoids (GCs) in fish. The optimized pretreatment conditions were examined. The target compounds were extracted with acetonitrile under the condition of Na2 EDTA-Mcllvaine buffer, and the usage of clean-up reagents was 25 mg anhydrous magnesium sulfate, 12. 5 mg octadecylsilane and 6. 25 mg N-Propylethylenediamine sorbent for extracted solvent of each milliliter. The positive results acquired by this high-throughput method were confirmed by liquid chromatography-tandem mass spectrometry ( LC-MS/MS) . The detection limit for these 59 drugs was in the range of 0. 5-5. 3 μg/kg. The method is time-saving, convenient, effective and wide coverage. Its sensitivity can meet the requirement of the detection of drug residues in aquatic products.