CAJ | 학술논문

利用仔-仔共轭效应,将1-芘丁酸( PBA)与导电高聚物聚3,4-乙撑二氧噻吩( PEDOT)相连,并通过Zr4+与羧基形成的配位键将羟基铁卟啉( Hematin)与PBA相连接,将Hematin固定于电极上,构建出一种制备过程简单的新型传感器( GCE/PEDOT/PBA/Hematin)。为了检验这种仿生传感器的稳定性和灵敏度,通过循环伏安法(CV)、交流阻抗法(EIS)和时间电流曲线法(i-t)等各种技术,将其应用于电化学检测中。 CV扫描证实在充分除氧的PBS缓冲液中,GCE/PEDOT/PBA/Hematin出现一对准可逆的氧化还原峰,其电子转移速率常数约为4.8 s-1,说明聚合于电极上的PEDOT膜增加了Hematin的电子转移速率。另外,通过i-t曲线检测邻苯二酚,在邻苯二酚浓度5×10-7~2×10-4mol/L范围内,其与催化电流强度呈线性相关,i=0.018C+0.006(R=0.9998),检测灵敏度为0.258μA (μmol·cm2),检出限为0.33 nmol/L(S/N=3),证明此仿生传感器稳定,简单且灵敏度高。
이용자-자공액효응,장1-비정산( PBA)여도전고취물취3,4-을탱이양새분( PEDOT)상련,병통과Zr4+여최기형성적배위건장간기철계람( Hematin)여PBA상련접,장Hematin고정우전겁상,구건출일충제비과정간단적신형전감기( GCE/PEDOT/PBA/Hematin)。위료검험저충방생전감기적은정성화령민도,통과순배복안법(CV)、교류조항법(EIS)화시간전류곡선법(i-t)등각충기술,장기응용우전화학검측중。 CV소묘증실재충분제양적PBS완충액중,GCE/PEDOT/PBA/Hematin출현일대준가역적양화환원봉,기전자전이속솔상수약위4.8 s-1,설명취합우전겁상적PEDOT막증가료Hematin적전자전이속솔。령외,통과i-t곡선검측린분이분,재린분이분농도5×10-7~2×10-4mol/L범위내,기여최화전류강도정선성상관,i=0.018C+0.006(R=0.9998),검측령민도위0.258μA (μmol·cm2),검출한위0.33 nmol/L(S/N=3),증명차방생전감기은정,간단차령민도고。
A new biosensor was prepared based on conductive polymer poly ( 3 , 4-ethylenedioxythiophene ) ( PEDOT) and 1-pyrenebutanoic acid ( PBA) through π-π stacking, as well as hematin associated with PBA through coordinate bonds of Zr4+ and carboxyl group. Its stability and sensitivity were examined by cyclic voltammetry ( CV) , electrochemical impedance spectroscopy ( EIS) and current-time ( i-t) method. A pair of well defined and quasi-reversible redox peaks was observed when GCE/PEDOT/PBA/hematin was tested by CV in PBS without oxygen. The electron transfer rate constant was estimated to be 4. 8 s-1 . Results indicated that the PEDOT film enhanced the electron transfer process of hematin. When the i-t method was used to detect the response of biosensor to catechol with different concentrations, it displayed a linear response for the reduction of catechol in the range of 0. 5-200 μmol/L. The linear equation was i=0. 018C+0. 006 ( R=0. 9998), and the detection sensitivity was 0. 258 μA (μmol/L· cm2) with a detection limit of 0. 33 nmol/L (S/N=3). The results illustrate that the GCE/PEDOT/PBA/hematin biosensor is very sensitive and stable.