CAJ | 학술논문

采用梯度稀释涂布选择性平板的方法从腐烂的桉树伐桩中分离筛选出一株产纤维素酶的真菌菌株，将其编号为F01。对菌株F01的羧甲基纤维素、滤纸和结晶纤维素酶活力进行了测定，其酶活力大小分别为4.00 IU/mL、0.12 IU/mL和0.10 IU/mL。形态和ITS序列鉴定菌株F01为木霉属真菌。系统发育分析结果表明，木霉属菌株F01和棘孢木霉T20为一个共同分枝，且进化距离较近。菌株F01纤维素酶最适pH和温度分别为4.5和55℃，酶活力在偏酸性条件下稳定且在30~55℃范围内具有一定的耐热性。木霉属真菌F01有较高的纤维素酶活力，其在加快桉树伐桩腐烂进程方面可能具有良好的应用前景。
채용제도희석도포선택성평판적방법종부란적안수벌장중분리사선출일주산섬유소매적진균균주，장기편호위F01。대균주F01적최갑기섬유소、려지화결정섬유소매활력진행료측정，기매활력대소분별위4.00 IU/mL、0.12 IU/mL화0.10 IU/mL。형태화ITS서렬감정균주F01위목매속진균。계통발육분석결과표명，목매속균주F01화극포목매T20위일개공동분지，차진화거리교근。균주F01섬유소매최괄pH화온도분별위4.5화55℃，매활력재편산성조건하은정차재30~55℃범위내구유일정적내열성。목매속진균F01유교고적섬유소매활력，기재가쾌안수벌장부란진정방면가능구유량호적응용전경。
Decayed Eucalyptus stumps was taken as the sample to screen the microbes which can accelerate the decay of stumps. Using gradient dilution and spread selective plate methods, a fungi strain F01 was screened. The CMCase, FPA and Avicelase of strain F01 were assyed, the results were 4.00 IU/mL, 0.12 IU/mL and 0.10 IU/mL, respectively. According to the morphology and ITS sequences analysis, strain F01 was belonged to Trichoderma sp.. The phylogenetic trees result showed that strain F01 have a common branch with Trichoderma asperellum T20 and their evolutionary distance was near. The optimum pH and temperature of cellulose produced by Trichoderma sp. F01 were 4.5 and 55℃, the enzyme activity was stable under the acidic situation and 30~55℃. Trichoderma sp. F01 have high cellulase activity and showed value of practical applications in accelerate the decay of Eucalyptus stumps.