中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2013年
16期
59-62
,共4页
黄鲛%易进海%刘玉红%黄志芳%陈燕%刘云华
黃鮫%易進海%劉玉紅%黃誌芳%陳燕%劉雲華
황교%역진해%류옥홍%황지방%진연%류운화
天南星%半夏%白附子%核苷%高效液相色谱
天南星%半夏%白附子%覈苷%高效液相色譜
천남성%반하%백부자%핵감%고효액상색보
Rhizoma Arisaema%Rhizoma Pinellie%Rhizoma Typhonii%nucleoside%HPLC
目的:建立HPLC测定天南星、半夏、白附子中8种核苷成分含量的方法.方法:采用Phenomenex Luna PFP(2)柱(4.6 mm ×250 mm,5 μm),以甲醇-水为流动相,梯度洗脱,流速0.8 mL· min-1,柱温35℃,检测波长260 nm.结果:尿嘧啶、次黄嘌呤、黄嘌呤、尿苷、肌苷、鸟苷、胸苷、腺苷进样量分别在0.00434～0.434 μg(r=0.9999),0.00234 ～0.234 μg(r=0.9999),0.00407 ～0.407μg(r=0.9997),0.01090 ～1.090μg(r=0.9999),0.00201 ～0.201 μg(r=0.9999),0.00732 ～0.732 μg(r=0.9999),0.00122 ～0.122 μg(r=0.9997),0.00207 ～0.207 μg(r=0.9999)有良好的线性关系;平均回收率分别为104.2％ (RSD 2.1％),98.7％ (RSD 2.4％),103.7％ (RSD 3.0％),99.4％ (RSD 1.4％),101.8％ (RSD 2.0％),102.9％ (RSD 1.9％),98.3％ (RSD 2.8％),102.3％ (RSD 2.7％).结论:方法简便、准确、快速,结果可靠,为测定天南星、半夏、白附子中8种核苷成分提供依据.
目的:建立HPLC測定天南星、半夏、白附子中8種覈苷成分含量的方法.方法:採用Phenomenex Luna PFP(2)柱(4.6 mm ×250 mm,5 μm),以甲醇-水為流動相,梯度洗脫,流速0.8 mL· min-1,柱溫35℃,檢測波長260 nm.結果:尿嘧啶、次黃嘌呤、黃嘌呤、尿苷、肌苷、鳥苷、胸苷、腺苷進樣量分彆在0.00434～0.434 μg(r=0.9999),0.00234 ～0.234 μg(r=0.9999),0.00407 ～0.407μg(r=0.9997),0.01090 ～1.090μg(r=0.9999),0.00201 ～0.201 μg(r=0.9999),0.00732 ～0.732 μg(r=0.9999),0.00122 ～0.122 μg(r=0.9997),0.00207 ～0.207 μg(r=0.9999)有良好的線性關繫;平均迴收率分彆為104.2％ (RSD 2.1％),98.7％ (RSD 2.4％),103.7％ (RSD 3.0％),99.4％ (RSD 1.4％),101.8％ (RSD 2.0％),102.9％ (RSD 1.9％),98.3％ (RSD 2.8％),102.3％ (RSD 2.7％).結論:方法簡便、準確、快速,結果可靠,為測定天南星、半夏、白附子中8種覈苷成分提供依據.
목적:건립HPLC측정천남성、반하、백부자중8충핵감성분함량적방법.방법:채용Phenomenex Luna PFP(2)주(4.6 mm ×250 mm,5 μm),이갑순-수위류동상,제도세탈,류속0.8 mL· min-1,주온35℃,검측파장260 nm.결과:뇨밀정、차황표령、황표령、뇨감、기감、조감、흉감、선감진양량분별재0.00434～0.434 μg(r=0.9999),0.00234 ～0.234 μg(r=0.9999),0.00407 ～0.407μg(r=0.9997),0.01090 ～1.090μg(r=0.9999),0.00201 ～0.201 μg(r=0.9999),0.00732 ～0.732 μg(r=0.9999),0.00122 ～0.122 μg(r=0.9997),0.00207 ～0.207 μg(r=0.9999)유량호적선성관계;평균회수솔분별위104.2％ (RSD 2.1％),98.7％ (RSD 2.4％),103.7％ (RSD 3.0％),99.4％ (RSD 1.4％),101.8％ (RSD 2.0％),102.9％ (RSD 1.9％),98.3％ (RSD 2.8％),102.3％ (RSD 2.7％).결론:방법간편、준학、쾌속,결과가고,위측정천남성、반하、백부자중8충핵감성분제공의거.
