临床儿科杂志
臨床兒科雜誌
림상인과잡지
2013年
8期
719-722
,共4页
卡他莫拉菌%耐药性%基因型
卡他莫拉菌%耐藥性%基因型
잡타막랍균%내약성%기인형
Moraxella catarrhalis%antimicrobial resistance%genotyping
目的调查儿童呼吸道卡他莫拉菌分离株产β内酰胺酶情况和耐药性，以及菌株BRO酶基因特征。方法2011年6月至2012年9月，从儿童呼吸道分离卡他莫拉菌401株；用微量肉汤稀释法测定常用抗生素最低抑菌浓度，用Nitroceifn纸片法检测β-内酰胺酶，用PCR扩增结合限制性内切酶分析方法对分离株进行BRO基因分型。结果401株卡他莫拉菌的β-内酰胺酶产酶率为96.5%（387/401）；产酶株对氨苄西林、头孢克洛、头孢呋辛的MIC50、MIC90和耐药率明显高于非产酶株，差异有统计学意义（P<0.05）；产酶株BRO基因阳性率为99.2%（384/387），其中BRO-1型为93.0%，BRO-2型为7.0%；BRO-1+菌株对氨苄西林、头孢克洛、头孢呋辛和阿齐霉素的MIC50和MIC90明显高于BRO-2+菌株。结论儿童呼吸道卡他莫拉菌分离株β-内酰胺酶产酶率高，产酶株主要携带BRO-1基因，其对部分β-内酰胺类和大环内酯类抗生素的影响明显高于BRO-2型菌株。
目的調查兒童呼吸道卡他莫拉菌分離株產β內酰胺酶情況和耐藥性，以及菌株BRO酶基因特徵。方法2011年6月至2012年9月，從兒童呼吸道分離卡他莫拉菌401株；用微量肉湯稀釋法測定常用抗生素最低抑菌濃度，用Nitroceifn紙片法檢測β-內酰胺酶，用PCR擴增結閤限製性內切酶分析方法對分離株進行BRO基因分型。結果401株卡他莫拉菌的β-內酰胺酶產酶率為96.5%（387/401）；產酶株對氨芐西林、頭孢剋洛、頭孢呋辛的MIC50、MIC90和耐藥率明顯高于非產酶株，差異有統計學意義（P<0.05）；產酶株BRO基因暘性率為99.2%（384/387），其中BRO-1型為93.0%，BRO-2型為7.0%；BRO-1+菌株對氨芐西林、頭孢剋洛、頭孢呋辛和阿齊黴素的MIC50和MIC90明顯高于BRO-2+菌株。結論兒童呼吸道卡他莫拉菌分離株β-內酰胺酶產酶率高，產酶株主要攜帶BRO-1基因，其對部分β-內酰胺類和大環內酯類抗生素的影響明顯高于BRO-2型菌株。
목적조사인동호흡도잡타막랍균분리주산β내선알매정황화내약성，이급균주BRO매기인특정。방법2011년6월지2012년9월，종인동호흡도분리잡타막랍균401주；용미량육탕희석법측정상용항생소최저억균농도，용Nitroceifn지편법검측β-내선알매，용PCR확증결합한제성내절매분석방법대분리주진행BRO기인분형。결과401주잡타막랍균적β-내선알매산매솔위96.5%（387/401）；산매주대안변서림、두포극락、두포부신적MIC50、MIC90화내약솔명현고우비산매주，차이유통계학의의（P<0.05）；산매주BRO기인양성솔위99.2%（384/387），기중BRO-1형위93.0%，BRO-2형위7.0%；BRO-1+균주대안변서림、두포극락、두포부신화아제매소적MIC50화MIC90명현고우BRO-2+균주。결론인동호흡도잡타막랍균분리주β-내선알매산매솔고，산매주주요휴대BRO-1기인，기대부분β-내선알류화대배내지류항생소적영향명현고우BRO-2형균주。
Objectives To investigate antimicrobial resistance and beta-lactamase production of Moraxella catarrhalis isolates from respiratory tract in children and to understand the characteristics of BRO beta-lactamase gene. Methods From June 2011 to Sep-tember 2012, 401 Moraxella catarrhalis isolates were obtained from respiratory tract in children. Minimum inhibitory concentrations (MIC) of commonly-used antibiotics were determined by microbroth dilution assay, and beta-lactamase production was detected by Nitroceifn disk test. PCR combining restriction endonuclease analysis was employed to do the BRO genotyping. Results 96.5%iso-lates were beta-lactamase positive (387/401), MIC (MIC50/MIC90) values and resistant rates of beta-lactamase producing isolates were higher than those of non beta-lactamase producing isolates for ampicillin, cefaclor and cefuroxime (P<0.05). The positive rate of BRO gene was 99.2%in beta-lactamase producing isolates (384/387), consisting of 93.0%BRO-1 isolates and 7.0%BRO-2 isolates. MIC50 and MIC90 values of BRO-1+isolates were higher than those of BRO-2+isolates for ampicillin, cefaclor, cefuroxime and azithromycin. Conclusions The beta-lactamase production rate is high in Moraxella catarrhalis isolates from respiratory tract in children. BRO-1 type was the dominant genotype of beta-lactamase producing isolates, having more inlfuence than BRO-2 type in the inlfuence on some beta-lactams and macrolides.
