中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
5期
595-598
,共4页
谷迪西%刘超%刘洋%肖建英
穀迪西%劉超%劉洋%肖建英
곡적서%류초%류양%초건영
蛋白激酶类/代谢%甲状腺肿瘤/病理学%癌,鳞状细胞/病理学%肿瘤细胞,培养的
蛋白激酶類/代謝%甲狀腺腫瘤/病理學%癌,鱗狀細胞/病理學%腫瘤細胞,培養的
단백격매류/대사%갑상선종류/병이학%암,린상세포/병이학%종류세포,배양적
Protein kinases/metabolism%Thyroid neoplasms/pathology%Carcinoma,squamous cell/pathology%Tumor cells,cultured
目的 研究蛋白激酶CK2α在人甲状腺鳞癌细胞SW579中的表达和活性,探讨其与甲状腺癌细胞之间的关系.方法 体外培养SW579和Hacat细胞,应用逆转录-聚合酶链反应(RT-PCR)和Western blotting技术分别检测蛋白激酶CK2α mRNA和蛋白在SW579细胞和Hacat细胞中的表达；应用放射自显影法检测细胞内蛋白激酶CK2的活性.结果 蛋白激酶CK2α mRNA和蛋白在SW579细胞中高表达,在Hacat细胞中低表达(mRNA:0.92±0.01 vs 0.52±0.02,t=19.24,P＜0.01；蛋白:0.98±0.01 vs 0.37±0.02,t=24.14,P＜0.01)；SW579细胞中CK2的活性高于Hacat细胞(4911±151.30 vs 1999±87.90,t=16.64,P＜0.01).结论 蛋白激酶CK2α过表达可能与甲状腺癌的发生和发展有关.
目的 研究蛋白激酶CK2α在人甲狀腺鱗癌細胞SW579中的錶達和活性,探討其與甲狀腺癌細胞之間的關繫.方法 體外培養SW579和Hacat細胞,應用逆轉錄-聚閤酶鏈反應(RT-PCR)和Western blotting技術分彆檢測蛋白激酶CK2α mRNA和蛋白在SW579細胞和Hacat細胞中的錶達；應用放射自顯影法檢測細胞內蛋白激酶CK2的活性.結果 蛋白激酶CK2α mRNA和蛋白在SW579細胞中高錶達,在Hacat細胞中低錶達(mRNA:0.92±0.01 vs 0.52±0.02,t=19.24,P＜0.01；蛋白:0.98±0.01 vs 0.37±0.02,t=24.14,P＜0.01)；SW579細胞中CK2的活性高于Hacat細胞(4911±151.30 vs 1999±87.90,t=16.64,P＜0.01).結論 蛋白激酶CK2α過錶達可能與甲狀腺癌的髮生和髮展有關.
목적 연구단백격매CK2α재인갑상선린암세포SW579중적표체화활성,탐토기여갑상선암세포지간적관계.방법 체외배양SW579화Hacat세포,응용역전록-취합매련반응(RT-PCR)화Western blotting기술분별검측단백격매CK2α mRNA화단백재SW579세포화Hacat세포중적표체；응용방사자현영법검측세포내단백격매CK2적활성.결과 단백격매CK2α mRNA화단백재SW579세포중고표체,재Hacat세포중저표체(mRNA:0.92±0.01 vs 0.52±0.02,t=19.24,P＜0.01；단백:0.98±0.01 vs 0.37±0.02,t=24.14,P＜0.01)；SW579세포중CK2적활성고우Hacat세포(4911±151.30 vs 1999±87.90,t=16.64,P＜0.01).결론 단백격매CK2α과표체가능여갑상선암적발생화발전유관.
Objective To investigate the expression of protein kinase CK2α in thyroid carcinoma SW579 cells and its significance.Methods SW579 cells and Hacat cells were cultured in vitro and the expression levels of protein kinase CK2α mRNA and protein in SW579 cells and Hacat cells were measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Moreover,CK2 activity was also measured.Results It was showed that the mRNA (0.92 ±0.01 vs 0.52 ±0.02,t =19.24,P ＜0.01)and protein(0.98 ±0.01 vs 0.37 ±0.02,t =24.14,P ＜0.01) expression of protein kinase CK2αwere significantly stronger in SW579 cells relative to Hacat cells.Conclusions Formation and development of thyroid carcinoma may be in connection with the overexpression of protein kinase CK2α.
