CAJ | 학술논문

本研究利用差异显示技术检测经低温处理的小麦品种“石新828”及对照材料中的mRNA，获得2条差异片段序列。经Blast比对后，发现其中一条长为293 bp的差异片段序列与小麦的一个冷调蛋白基因的mRNA (GenBank：AB097412.1)同源性为99%。该基因编码的蛋白属于磷脂酰乙醇胺结合蛋白家族，命名为wpebp。经荧光定量PCR分析，wpebp基因的表达量随低温处理时间的增长总体呈上升趋势，表明该基因与小麦的抗寒能力相关。
본연구이용차이현시기술검측경저온처리적소맥품충“석신828”급대조재료중적mRNA，획득2조차이편단서렬。경Blast비대후，발현기중일조장위293 bp적차이편단서렬여소맥적일개랭조단백기인적mRNA (GenBank：AB097412.1)동원성위99%。해기인편마적단백속우린지선을순알결합단백가족，명명위wpebp。경형광정량PCR분석，wpebp기인적표체량수저온처리시간적증장총체정상승추세，표명해기인여소맥적항한능력상관。
We obtained 2 sequences of differential fragment in cold-induced wheat variety“Shi Xin 828”using dif-ferential display method. After Blast analysis, it was indicated that the homology between 293 bp differential frag-ment and mRNA of wheat cold-regulated gene (GenBank: AB097412.1) is 99%. The protein belongs to phos-phatidylethanolamine binding proteins and it was called wpebp. The fluorescence quantitative PCR analysis showed that there was an upward trend in wpebp gene expression quantities as cold-induced time extended. We could con-clude that the gene is related to cold resistance of the wheat.