CAJ | 학술논문

本文运用RT-PCR方法克隆了黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus, CGMMV)湖南邵阳株系的基因组MP (CGMMV-HuNSY movement protein)片段，测序并进行了分析。结果显示，片段全长共有803 bp (KC684977)，编码由264个氨基酸组成的蛋白质，推测分子量约28.87 kD，理论等电点pI为9.06，ProtParam预测显示为不稳定蛋白，与已报道的辽宁分离物病毒的MP作比较，核苷酸相似性为99.6%，氨基酸相似性为98.9%；湖南邵阳株系CGMMV MP蛋白无高度卷曲螺旋部位，有跨膜结构区域，该部位表现为疏水性，可能为蛋白互作位点；磷酸化位点均匀分布于整个多肽链中，存在5个主要的B细胞抗原表位预测位点；对烟草花叶病毒属病毒MP氨基酸序列进行了motif查找，发现了该属病毒氨基酸序列的3个保守区段，还进行了密码子偏向性分析。此外，发现了1个酰胺化位点和1个依赖于cAMP和cGMP的蛋白激酶磷酸化位点，可能与病毒的侵染机制有关。
본문운용RT-PCR방법극륭료황과록반박화협병독(cucumber green mottle mosaic virus, CGMMV)호남소양주계적기인조MP (CGMMV-HuNSY movement protein)편단，측서병진행료분석。결과현시，편단전장공유803 bp (KC684977)，편마유264개안기산조성적단백질，추측분자량약28.87 kD，이론등전점pI위9.06，ProtParam예측현시위불은정단백，여이보도적료녕분리물병독적MP작비교，핵감산상사성위99.6%，안기산상사성위98.9%；호남소양주계CGMMV MP단백무고도권곡라선부위，유과막결구구역，해부위표현위소수성，가능위단백호작위점；린산화위점균균분포우정개다태련중，존재5개주요적B세포항원표위예측위점；대연초화협병독속병독MP안기산서렬진행료motif사조，발현료해속병독안기산서렬적3개보수구단，환진행료밀마자편향성분석。차외，발현료1개선알화위점화1개의뢰우cAMP화cGMP적단백격매린산화위점，가능여병독적침염궤제유관。
The genome segment of MP (movement protein) of cucumber green mottle mosaic virus of hunan Shaoyang isolate was cloned by using the RT-PCR method, sequencing and analysis. The result show that the MP had 803 nucleotides (KC684977) encoding a polypeptide of 264 amino acids with a molecular weight of 28.87 kD and an isoelectric point of 9.06, it was predicted that the protein as unstable by ProtParam. Compared with Liaoning isolates, the similarities of nucleotide and amino acid respectively are 99.6%and 98.9%. There are not highly coiled coil regions on CGMMV MP protein, but find transmembrane helices with hydrophobic and it is a potential protein interaction sites, Phosphorylation sites uniform distribution in the whole of the polypeptide chain, there are five major B cell antigen epitope prediction sites. By searching the motif of tobamo virus MP amino acids, we found that three conservative sections, and amino acids exist codon bias. Moreover, we found a amidation site and a cAMP-and cGMP-dependent protein kinase phosphorylation site, which may involved in virus infection mechanism.