CAJ | 학술논문

本文用不同的供体细胞、去核方法以及TSA处理浓度研究其对巴马小型猪手工克隆胚发育的影响，试验结果发现：克隆胚的发育率在相同代次的不同供体细胞之间，极体定向和化学辅助去核法之间均无显著性差异(p>0.05)；TSA浓度为40 nmol/L时克隆胚的囊胚率最高，但与对照组相比无显著性差异(p>0.05)。这说明三种供体细胞均能支持巴马小型猪克隆胚的早期发育，化学辅助和极体定向去核法均为较好的去核方法。
본문용불동적공체세포、거핵방법이급TSA처리농도연구기대파마소형저수공극륭배발육적영향，시험결과발현：극륭배적발육솔재상동대차적불동공체세포지간，겁체정향화화학보조거핵법지간균무현저성차이(p>0.05)；TSA농도위40 nmol/L시극륭배적낭배솔최고，단여대조조상비무현저성차이(p>0.05)。저설명삼충공체세포균능지지파마소형저극륭배적조기발육，화학보조화겁체정향거핵법균위교호적거핵방법。
The cloned embryos were constructed with different donor cells, enucleation methods and then treat-ment with different concentration of TSA in order to evaluate their potential effects on the development of Guangxi Bama Minipig handmade cloned embryos in vitro. It was showed that the development rate of handmade cloned embryos were not only similar between the three groups of cell types with the same generation, but also be-tween the chemically assisted handmade enucleation (CAHE) and polar body-oriented-handmade enucleation (OHE) groups (p>0.05). Although the blastocyst rate in the 40 nmol/L TSA group was higher, there was no significant dif-ference among the control and other groups. It is concluded that the three kinds of donor cells could well support in vitro embryonic development of Bama minipig handmade cloned embryos, CAHE and OHE could both be the enucleation method for handmade cloning.