CAJ | 학술논문

通过黄连浸出液对脑缺血小鼠灌胃，测定海马区细胞总蛋白和SOD的含量变化，探讨黄连对缺血性脑损伤的保护和修复作用。对36只小鼠随机分为实验组、对照组和正常组，以黄连水浸出液(0.1 mg/g)灌胃实验组，分别于不同时间段取小鼠海马组织制备组织匀浆，采用BCA法和WST-1法分别测总蛋白含量与超氧化物歧化酶(SOD)活力。结果显示实验组海马组织中的总蛋白含量和超氧化物歧化酶(SOD)活力高于对照组低于正常组(p约0.05)；在24 h时间段实验组两项指标与对照组差距达最高值。由此推测黄连浸出液对提高小鼠脑缺血海马组织总蛋白含量和SOD活力有一定的作用。
통과황련침출액대뇌결혈소서관위，측정해마구세포총단백화SOD적함량변화，탐토황련대결혈성뇌손상적보호화수복작용。대36지소서수궤분위실험조、대조조화정상조，이황련수침출액(0.1 mg/g)관위실험조，분별우불동시간단취소서해마조직제비조직균장，채용BCA법화WST-1법분별측총단백함량여초양화물기화매(SOD)활력。결과현시실험조해마조직중적총단백함량화초양화물기화매(SOD)활력고우대조조저우정상조(p약0.05)；재24 h시간단실험조량항지표여대조조차거체최고치。유차추측황련침출액대제고소서뇌결혈해마조직총단백함량화SOD활력유일정적작용。
Through using the Coptis lixivium to gavage the mice with cerebral ischemia, we determine the change of total protein content and SOD from hippocampus cells, in order to discuss the function of Coptis to protect and repair of ischemic brain injury. 36 mice were randomly divided into experimental group, control group and normal group, After using Coptis lixivium (0.1 mg/g) to gavage the mice in experimental group, we prepare the tissue homogenates of mouse hippocampus at different time periods, and use the BCA method and the WST-1 assay to measure total protein content and superoxide dismutase (SOD) activity. The results showed that the mice hippocam-pus of the total protein content and superoxide dismutase (SOD) activity in experimental group is higher than the control group, but it is lower than normal group (p<0.05). The gaps of two indicators from comparing the experimen-tal group with the control group, is up to the highest value in the 24 h time period. Through this, we can infer that the Coptis lixivium have the function to improve total protein content and SOD of the mice hippocampus with cerebral ischemia.