CAJ | 학술논문

芋疫病是广西芋头上的重要病害。本研究从桂东南地区部分乡镇的芋头产区采集并分离获得12个芋疫霉菌株，通过与辣椒疫霉A1交配型HD-3菌株和A2交配型S-5菌株对峙培养来测定其交配型，并以ITS1/ITS4通用引物对基因组DNA进行PCR扩增，得到各菌株的ITS序列。结果表明，通过对ITS序列的Blast比对，发现所有菌株的ITS序列与GenBank中登录的芋疫霉(Phytophthora colocasiae)不同分离物序列同源性达97%~100%，证明所采样本均为芋疫霉。从桂东南地区采集到的12个芋疫霉菌株均为A2交配型，未发现其它交配型菌株。
우역병시엄서우두상적중요병해。본연구종계동남지구부분향진적우두산구채집병분리획득12개우역매균주，통과여랄초역매A1교배형HD-3균주화A2교배형S-5균주대치배양래측정기교배형，병이ITS1/ITS4통용인물대기인조DNA진행PCR확증，득도각균주적ITS서렬。결과표명，통과대ITS서렬적Blast비대，발현소유균주적ITS서렬여GenBank중등록적우역매(Phytophthora colocasiae)불동분리물서렬동원성체97%~100%，증명소채양본균위우역매。종계동남지구채집도적12개우역매균주균위A2교배형，미발현기타교배형균주。
Taro leaf blight is an important disease in Guangxi province. Disease samples were collected from different regions in south-eastern Guangxi. A total of 12 isolates of Phytophthora colocasiae were isolated from the infected taro leaves. Confront culture method was used to detect P. colocasiae mating types with P. capsici A1 (HD-3) and A2 (S-5) mating types. ITS sequences of different isolates were determined by sequencing PCR products generated with genomic DNA of the isolates as templates and universal ITS1/ITS4 primers. Blast results revealed that the isolates shared 97%~100%sequence homology to isolates of P. colocasiae in the GenBank. All 12 isolates of P. colocasiae belonged to A2 mating type.