热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
1期
59-62
,共4页
尖孢镰刀菌古巴专化型%尖孢镰刀菌萎蔫专化型%绿色荧光蛋白%原生质体转化
尖孢鐮刀菌古巴專化型%尖孢鐮刀菌萎蔫專化型%綠色熒光蛋白%原生質體轉化
첨포렴도균고파전화형%첨포렴도균위언전화형%록색형광단백%원생질체전화
Fusarium oxysporum f. sp. Cubence%Fusarium oxysporum f. sp. Vasinfectum%green fluorescent protein%protoplast transformation
利用PEG介导的原生质体转化方法，将绿色荧光蛋白基因转入到6株香蕉枯萎病菌和1株棉花枯萎病菌中。结果表明：转化子连续转接5代能够稳定遗传，荧光强度良好， PCR验证gfp基因已转入到菌株中，为后续研究香蕉枯萎病菌和棉花枯萎病菌的侵染、防治等提供可视化的检测和分析手段。
利用PEG介導的原生質體轉化方法，將綠色熒光蛋白基因轉入到6株香蕉枯萎病菌和1株棉花枯萎病菌中。結果錶明：轉化子連續轉接5代能夠穩定遺傳，熒光彊度良好， PCR驗證gfp基因已轉入到菌株中，為後續研究香蕉枯萎病菌和棉花枯萎病菌的侵染、防治等提供可視化的檢測和分析手段。
이용PEG개도적원생질체전화방법，장록색형광단백기인전입도6주향초고위병균화1주면화고위병균중。결과표명：전화자련속전접5대능구은정유전，형광강도량호， PCR험증gfp기인이전입도균주중，위후속연구향초고위병균화면화고위병균적침염、방치등제공가시화적검측화분석수단。
Several Fusarium oxysporum strains (Foc1, Foc4, Foc 8359#, Foc 8361#, Foc 8363#, Foc 8368# and Fov) have been successfully transformed with the gene coding for green fluorescent protein (GFP) using protoplast transformation method mediated by PEG. Transformants can emit quite stable fluorescence under 480 nm laser after five successive subcultures. PCR analysis confirmed that the gfp gene had been transformed into F. oxysporum strains. Those GFP transformations of F. oxysporum will provide a visible material for the study of infection and control of fusarium wilt of banana and cotton.
