CAJ | 학술논문

试验以公农1号紫花苜蓿种子为实验材料，采取不同的消毒处理，选取子叶、叶片、胚轴为外植体，研究了不同消毒方式、不同外植体对公农l号紫花苜蓿品种再生的影响。结果表明：种子在消毒前，经无菌水浸泡24h（预处理）比不浸泡的消毒效果好，但是浸泡后，用酒精消毒时间不可过长；2％次氯酸钠、10％次氯酸钙、0．1％升汞三种消毒剂中，0．1％升汞消毒效果最好，种子带菌率为0％，75％的乙醇预处理后可大幅降低种子的带茵率；三种消毒剂中10％次氯酸钙一无水处理的紫花苜蓿种子发芽率最高；三种外植体均容易形成愈伤组织，经过综合比较，选取胚轴为公农1号紫花苜蓿品种的最佳外植体。
시험이공농1호자화목숙충자위실험재료，채취불동적소독처리，선취자협、협편、배축위외식체，연구료불동소독방식、불동외식체대공농l호자화목숙품충재생적영향。결과표명：충자재소독전，경무균수침포24h（예처리）비불침포적소독효과호，단시침포후，용주정소독시간불가과장；2％차록산납、10％차록산개、0．1％승홍삼충소독제중，0．1％승홍소독효과최호，충자대균솔위0％，75％적을순예처리후가대폭강저충자적대인솔；삼충소독제중10％차록산개일무수처리적자화목숙충자발아솔최고；삼충외식체균용역형성유상조직，경과종합비교，선취배축위공농1호자화목숙품충적최가외식체。
Medicago sativa L.CV. ＂Gongnong No.l＂ was used as experiment material, and the influence of regenerate by different disinfection methods, different explants （select the cotyledons, leaves, hypocotyls as explants） . The results showed that sterilization of seeds soaked in aseptic wat.er for 24 hours was better than that without soaking. However, after soaking with alcohol disinfectant is not long. Under constant temperature, the sterilization effects of three germicides （2% sodium hypochlorite, 10% calcium hypochlorite and l%v HgCI2） on alfalfa seeds were studied. The results showed that 1%~ HgCI2 had the greatest sterilization effect. The germ-bearing rate of seeds treated with 1%~ HgC12 was 0%. Sterilization of seeds soaked in 75% ethanol before seed sterilization decreased germ-bearing rate. The highest germination rate was observed in treatment with 1%~ HgCI2. The results showed that hypocotyl was to induce callus, was the most effective explants in the three explants.