CAJ | 학술논문

试验旨在研究不同微生物制剂处理对稻草瘤胃体外发酵指标的影响，试验选用A、B、C3种微生物制剂及其组合对稻草进行微贮试验，设7个处理组和1个对照组，稻草微贮期为60 d。采用体外批次培养方法测定其产气量、NH3-N、BCP浓度并进行相关性分析。结果表明：①微生物制剂A、B、C及其组合处理组的产气量高于对照组，单独使用以A制剂总产气量最大，联合使用以A+B组合总产气量最大；②除微生物制剂处理A组外，3种微生物制剂及其组合处理组NH3-N浓度均高于对照组，并且微生物制剂C及A+B+C组合显著高于对照组。而且均在瘤胃微生物生长最适的NH3-N浓度范围内；③3种微生物制剂及其组合处理组BCP浓度均高于对照组，并且微生物制剂A+B+C组合效果最好；④从NH3-N、BCP浓度综合考虑，单独使用以C制剂最好，联合使用以A+B+C组合较好；⑤培养液中BCP浓度与NH3-N浓度呈现中等相关，其回归方程为：Y=1.214X+10.232（Y为培养液中BCP浓度，X为为培养液中NH3-N浓度，R2=0.6778）
시험지재연구불동미생물제제처리대도초류위체외발효지표적영향，시험선용A、B、C3충미생물제제급기조합대도초진행미저시험，설7개처리조화1개대조조，도초미저기위60 d。채용체외비차배양방법측정기산기량、NH3-N、BCP농도병진행상관성분석。결과표명：①미생물제제A、B、C급기조합처리조적산기량고우대조조，단독사용이A제제총산기량최대，연합사용이A+B조합총산기량최대；②제미생물제제처리A조외，3충미생물제제급기조합처리조NH3-N농도균고우대조조，병차미생물제제C급A+B+C조합현저고우대조조。이차균재류위미생물생장최괄적NH3-N농도범위내；③3충미생물제제급기조합처리조BCP농도균고우대조조，병차미생물제제A+B+C조합효과최호；④종NH3-N、BCP농도종합고필，단독사용이C제제최호，연합사용이A+B+C조합교호；⑤배양액중BCP농도여NH3-N농도정현중등상관，기회귀방정위：Y=1.214X+10.232（Y위배양액중BCP농도，X위위배양액중NH3-N농도，R2=0.6778）
This experiment was conducted to study the effect of different microbial preparation or combination on rumen disappearance rate of rice straw in Jinjiang cattle. Microbial prepara-tion of A, B, C and combinations were selected in this experiment for micro-storage rice straw, the test is divided into six treatment groups and control group. Period of micro-storage rice straw is 60 days.By culture in vitro,gas production, NH3-N, BCP concentration of rice straw culture, was measured, and that correlation of it was analysis. The results showed that: ① Gas production of rice straw in the A、C、and A+B groups of microbial preparation was higher than that in control group, and that of A group was significantly higher than that in control group(P<0.05). Gas production of A group is maximum among single microbial preparation, and that of A+B group is maximum among different combinations. ② NH3-N concentration of rice straw in the groups of microbial preparation was higher than that in control group, and that of C and A+B+C group was higher than that in control group(P<0.05), and that of every groups is suitable for the growth of the rumen microbes. ③BCP concentration of rice straw in the groups of microbial preparation was higher than that in control group, and A+B+C group is best among different combinations. ④ By compre-hensive consideration concentration of NH3-N, BCP, C group is best among single micro-bial preparation, and A+B+C group is best among different combinations. ⑤ Correlation of BCP concentration is middle to that of NH3-N. Regression equation of it is that: Y=1.214X+10.232(Y: BCP concentration in culture liquid,X: NH3-N concentration in culture liquid,R2=0.677 8).