CAJ | 학술논문

目的探讨坎地沙坦酯（CAN）干预对大鼠急性心肌梗死（AMI）后内皮功能及梗死心肌胞外信号调节激酶（ERK） mRNA和 cAMP反应元件结合蛋白（CREB）mRNA表达的影响。方法30只动物被随机分为CAN组、假手术（Sham）组和 AMI组。干预2周后，测量血压、血管紧张素Ⅱ（AngⅡ）、血一氧化氮合酶（NOS）、一氧化氮（NO）的水平、离体胸主动脉舒缩功能、梗死面积及 ERK、CREB的mRNA在梗死区心肌中的表达水平。结果 AMI组较 Sham组血浆 AngⅡ水平显著升高、血清 NO、NOS水平明显降低。同时内皮依赖性舒张（EDD）功能减退、梗死区心肌 ERK、CREB的 mRNA表达明显增加。CAN治疗后，血清 NO、NOS水平升高至 Sham组水平，同时升高血浆 AngⅡ水平、缩小心肌梗死面积、降低梗死区心肌中 ERK、CREB的 mRNA表达，伴 EDD功能明显改善，但组间血压并无明显差别。结论 CAN显著改善 AMI后内皮功能紊乱（ED），缩小心肌梗死面积，降低 AMI大鼠心肌 ERK、CREBm RNA表达。
목적탐토감지사탄지（CAN）간예대대서급성심기경사（AMI）후내피공능급경사심기포외신호조절격매（ERK） mRNA화 cAMP반응원건결합단백（CREB）mRNA표체적영향。방법30지동물피수궤분위CAN조、가수술（Sham）조화 AMI조。간예2주후，측량혈압、혈관긴장소Ⅱ（AngⅡ）、혈일양화담합매（NOS）、일양화담（NO）적수평、리체흉주동맥서축공능、경사면적급 ERK、CREB적mRNA재경사구심기중적표체수평。결과 AMI조교 Sham조혈장 AngⅡ수평현저승고、혈청 NO、NOS수평명현강저。동시내피의뢰성서장（EDD）공능감퇴、경사구심기 ERK、CREB적 mRNA표체명현증가。CAN치료후，혈청 NO、NOS수평승고지 Sham조수평，동시승고혈장 AngⅡ수평、축소심기경사면적、강저경사구심기중 ERK、CREB적 mRNA표체，반 EDD공능명현개선，단조간혈압병무명현차별。결론 CAN현저개선 AMI후내피공능문란（ED），축소심기경사면적，강저 AMI대서심기 ERK、CREBm RNA표체。
Objective To study the interventional effects of Candesartan Cilexetil on vascular endothelial function and expres-sions of ERK/CREB signaling pathway in rats with acute myocardial infarction.Methods 30 rats were randomized into CAN group,AMI group and Sham group.Two weeks after the treatment rats were sacrificed and blood pressure,levels of blood AngⅡ, NO and NOS,endothelial vasomotor effects of isolated thoracic aorta strips,myocardial infarctual area and the expressions of ERK mRNA and CREB mRNA in infarctual myocardium were explored.Results Blood AngⅡ levels were distinctly higher in AMI group when compared with the Sham group,while the NO and NOS level were much lower.accompanied with EDDs decreased seri-ously,and higher mRNA expressions of ERK1 and CREB in infarctual myocardium.All of the above differences were significant. After the treatment of Candesartan Cilexetil,levels of serum NO and activities of NOS were obviously higher and almost reached the similar levels to those in Sham group,additionally endothelium-dependent diastole in isolated aortic strips improved greatly.Mean-while Candesartan Cilexetil can not only increase the plasma AngⅡ,but also decrease the size of myocardial infarction and the mR-NA expressions of ERK1 and CREB in infarctual myocardium significantly.However,blood pressure in all groups was not affected. Conclusion Candesartan Cilexetil can greatly improve the disordered endothelial function developing post-AMI,decrease the size of myocardial infarction and down-regulate the mRNA expressions of ERK and CREB in myocardium of infarctual zone.And all of the above protective effects of Candesartan Cilexetil were independent on blood pressure lowering.